【摘要】 通过对出雏率、HI抗体水平、外周血液中 ANAE+T细胞百分率、淋巴细胞转化率和免疫保护力的测定 ,评定了鸡新城疫胚胎免疫的不同免疫剂量和接种方法的效果。结果表明 ,3倍剂量免疫组的出雏率较低 (89.4% ) ,而 2倍剂量的 2个免疫组的出雏率均较高 (96 .9%、99.2 % ) ,接近非免疫对照组 (10 0 % ) ;2倍剂量旋转组雏鸡的 HI抗体于 14日龄时即达峰值 (7.9log2 ) ,到 42日龄时 ,其 HI抗体的水平仍然较高 (6 .5 log2 ) ,而 3倍剂量组和 2倍剂量非旋转组雏鸡的 HI抗体达到峰值的时间为 2 1日龄 ,到 42日龄时的 HI抗体水平 (分别为 4.0 log2和 3.5 log2 )较旋转组低 ;2倍剂量接种旋转组雏鸡于 14日龄 ANAE+T细胞率和淋巴细胞转化率均达到峰值 ,而 2倍剂量非旋转组和 3倍剂量组于 2 1日龄达到峰值 ,且与旋转组之间差异显著 ,试验组均与对照组之间差异显著。 5 0日龄攻毒时 ,3倍剂量免疫组的保护率较低 (78.9% ) ,2倍剂量免疫组的保护率较高 (96 .4% ) ,而旋转组的保护率最高 (10 0 % )。上述结果表明 ,选择 2倍剂量接种并加以适当地旋转进行免疫 ,可取得较好的免疫效果。更多还原

【Abstract】 In this paper,the immunological efficiency of different doses and ways were compared respectively with the hatchability,HI antibody level,the percentage of ANAE + T cells and lymphocyte blastogenesis rate and protection rate.The results showed that the hatchability in the three chicken dose group was the lowest(89.4%),and the two chicken dose group higher(96.9%,99.2%).HI antibody level in the two chicken doses with rotation group was higher(7.9 log2 at 21 days and 6.5 log2 at 42 days),while the non-rotation group low and the three doses group lowest.The results also indicated that at 14 days age the percentage of ANAE + T cells and lymphocyte blastogenesis rate in two chicken doses group with rotation(Ⅲ group) reached their top value,those of two chicken doses group with non-rotation(Ⅰ group) and three chicken doses group(Ⅱ group) at 21 days age.After challenge at 50 days age the protection rate of three doses group was low(78.9%),the non-rotation group higher(96.4%) and the rotation group highest(100%).The above results showed that the immunological effect of the two chicken doses is better.And the way of rotation at once after injection of vaccine may improve the effect.更多还原