【摘要】 目的 :探讨人血管内皮细胞在等离子体处理后的丝素蛋白膜表面的生长情况。方法 :等离子体由气体射频放电产生 ,工作气体为SO2 和NH3;材料表面元素进行XPS分析 ;用MTT法测定人脐静脉内皮细胞系HU VEC细胞生长 ;用间接免疫荧光法检测该细胞内Ⅷ凝血因子相关抗原的表达。结果 :SO2 和NH3 等离子体处理后 ,丝素蛋白膜表面分别被磺酸化和氨基化 ,并均能促进HUVEC细胞生长 ,而且对细胞形态和产生Ⅷ凝血因子的功能没有明显的影响。结论 :等离子体处理的丝素蛋白可以作为人内皮细胞的一种良好的培养基质。更多还原

【Abstract】 Object:To investigate the growth of human endothelial cells on the culture matrix of plasma-treated silk fibroin. Methods:Plasma was produced by gas discharge at radio frequency with SO 2 or NH 3 as working gas. XPS analysis was carried out to examine the concentratio of surface element. The growth of a human umbilical vein endothelial cell line (HUVEC) was determined by the MTT reagent-based colorimetric assay. Cellular coagulation factor Ⅷ was detected by indirect immunofluorescence method. Results: Treatment with SO 2 and NH 3 plasma was able to add the sulfonate and amino groups to the surface of silk fibroin membrane, and the growth of HUVEC cells could be accelerated on the silk fibroin matrix treated by both kinds of plasma, while the cell′s morphology and production of coagulation factor Ⅷ remained unchanged. Conclusion: Plasma-treated silk fibroin could be used as a good matrix for human endothelial cell culture.更多还原