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卵磷脂类保健食品中PE、PC的高效液相色谱测定
A study on determination of major phospholipid classes in lecithin dietary supplements by high-performance liquid chromatography
【摘要】 为准确快速地对卵磷脂保健食品中有效成分进行定量分析 ,我们建立了卵磷脂保健食品中磷脂主要组分磷脂酰胆碱 (PC)、磷脂酰乙醇胺 (PE)的高效液相色谱测定方法。方法的日间精密度为 5 4 %~ 9 0 %;PE、PC的加标回收率分别为 83 0 %~ 10 5 6 %和 81 3%~ 86 0 %,均可满足卫生分析的要求。对 5种卵磷脂保健食品中的PC、PE的含量进行了测定 ,PC、PE的含量分别为15 2 %~ 2 4 2 %和 3 1%~ 6 1%。方法的样品前处理方法简单 ,且检测灵敏度高 ,分析速度快 ,适于在国内推广使用
【Abstract】 A high\|performance liquid chromatography method for the quantification of major phospholipid classes\|phosphatidylcholine (PC),phosphatidylethanolamine (PE) in lecithin dietary supplements was described in order to determine their effective constituent accurately and rapidly.The method precision of PC and PE ranged from 5 4% to 9 0%.The recoveries for PC and PE ranged from 81 3% to 86 0% and from 83 0% to 105 6% respectively.5 kind of lecithin dietary supplements samples were measured under the optimized liquid chromatographic condition.The content of PC and PE ranged from 15 2% to 24 2% and from 3 1% to 6 1% respectively.The method described in the present study is simple,rapid and accurate,it could be recommended as a routine analytical method.
【Key words】 Chromatography,High Perssure Liquid; Phosphatidylcholines; Phosphatidylethanolamines; Dietary Supplements;
- 【文献出处】 中国食品卫生杂志 ,Chinese Journal of Food Hygiene , 编辑部邮箱 ,2002年04期
- 【分类号】O657.7
- 【被引频次】17
- 【下载频次】291