【摘要】 通过建立脑不对称动物模型 ,探讨脑不对称性对小鼠单核巨噬细胞产生炎性因子的影响。用伸爪取食法区分出左利、右利、双利小鼠 ,细菌脂多糖 (lipopolysaccharide ,LPS)体内刺激 2h后取小鼠腹腔巨噬细胞在体外LPS刺激下培养 ,2 4h后收集培养上清测定巨噬细胞产生白细胞介素 (interlukine 1β,IL 1β)、一氧化氮 (nitricox ide ,NO)水平。体内经LPS刺激的小鼠双利组和右利组IL 1(水平均显著高于左利组 ,双利组IL 1(水平高于右利组 ;未经体内LPS刺激的三组小鼠腹腔巨噬细胞产生NO水平相似 ,经体内LPS刺激的小鼠腹腔巨噬细胞产生NO的量为双利 >右利 >左利 ,其中双利组NO水平明显高于左利组 (P <0 .0 1)。脑不对称可影响下丘脑 垂体 肾上腺 单核巨噬细胞 (HPA MO/Mφ)调节环路。更多还原

【Abstract】 The interactions of cytokines,macrophage and hypothalamic pituitary adrenal (HPA) axis associated with lateralization were investigated. Paw preference test was used to select right pawed , left pawed, ambidextrous mice. Two weeks after the session of paw preference testing, mice were injected intraperitonally with 5 μg LPS dissolved in 0.5 mL of saline. The control groups were injected with 0.5 mL saline. Animals were killed by decapitation 2 h later. Peritoneal macrophages were collected and cultured. In the supernatants of cultured macrophage of the control Balb/c mice , the right pawed mice had a higher IL 1β level than left pawed mice( P< 0.05),while IL 1β of ambidextrous was higher than that of left pawed also; No difference of NO level in supernatants of macrophage culture was observed in the control mice. For the previously LPS injected mice, IL 1β level was higher in right pawed and ambidextrous than in left pawed mice( P< 0.05 ), while IL 1β of ambidextrous was the highest among them. NO level in the supernatants of cultured macrophages was significantly higher in ambidextrous than in left pawed mice( P< 0.05). The results indicated that the activities of HPA M O /Mφ were correlated to brain lateralization.更多还原