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高效液相色谱法测定颐神复记胶囊中二苯乙烯苷含量
Determination of 2,3,5,4′-tetrahydroxystibene-2-O-β-D-glucoside in Yishenfuji Capsule by HPLC
【摘要】 目的 :对颐神复记胶囊中二苯乙烯苷进行含量测定。方法 :高效液相色谱法 ,流动相为乙腈 水 (14∶86 ) ,紫外检测波长 32 0nm ,流速 0 8ml min。结果 :二苯乙烯苷在 0 0 5 0 8~ 0 2 5 4 μg范围内线性关系良好 ,平均回收率为 97 0 %。结论 :该法简单 ,分离完全 ,重现性好 ,可作为本制剂质量控制的方法
【Abstract】 Objective:To determine of contents of 2,3,5,4′\|tetrahydroxystibene\|2\|O\|β\|D\|glucoside in Yishenfuji Capsule;Methods:HPLC was adopted.The mobile phase was consisted of acetonitrile\|water(14∶86).The detection wavelength was at 320nm and flow rate was 0\^8ml/min.Results:The linearity was obtained over the range of 0\^0508~0 254μg.The average recovery was 97\^0%.Conclusion:The method was simple and reliable.It is suitable for the determination of 2,3,5.4′\|tetrahydroxystibene\|2\|O\|β\|D\|glucoside in Yishenfuji Capsule.
【关键词】 颐神复记胶囊;
二苯乙烯苷;
HPLC;
【Key words】 Yishenfuji Capsule; 2,3,5,4′\|tetrahydroxystibene\|2\|O\|β\|D\|glucoside; HPLC;
【Key words】 Yishenfuji Capsule; 2,3,5,4′\|tetrahydroxystibene\|2\|O\|β\|D\|glucoside; HPLC;
- 【文献出处】 中国实验方剂学杂志 ,Chinese Journal of Experimental Traditional Medical Formulae , 编辑部邮箱 ,2002年06期
- 【分类号】R286
- 【被引频次】3
- 【下载频次】39