【摘要】 从 1999年 10月至 2 0 0 1年 3月对导致栉孔扇贝 (Chlamysfarreri)大规模死亡的可疑病原进行了系统调查 ,在栉孔扇贝体内发现 1种细胞内寄生原核生物。根据该原核生物超微形态结构及所形成的包涵体形态特征及染色性质分析 ,初步确定为类立克次体 (Rickettsia likeorganisms ,RLO)。该RLO大小为 (3.6 2 3± 1.4 35 ) μm× (1.343± 0 .32 6 ) μm (n =4 5 ) ,主要寄生在栉孔扇贝的鳃、消化腺的上皮组织中。其感染率和感染强度与水温及栉孔扇贝死亡率呈负相关关系。人工感染试验证明 ,RLO可引起栉孔扇贝感染 ,但不形成大面积明显的组织病理变化。本研究表明 ,RLO对栉孔扇贝不具明显的致病性 ,不是导致栉孔扇贝大规模死亡的主要原因。更多还原

【Abstract】 A systematic investigation on the pathogen leading to the high mortality of scallop Chalmys farereri was carried out from October 1999 to March 2001. An intracellular prokaryotic organism was found in the cells of C. farreri which was determined as Rickettsia like organism based on the ultrastructure and morphology and the stain characteristics of their inclusions. The RLOs were in the shape of long rod with the average size of (3.623±1.435) μm×(1.343±0.326) μm (n=45)and usually parasitically settled inside the epithelial cells of gill and digestive gland. The prevalence and infecting intensity of RLO were in negatively correlation with water temperature and mortalities of C. farreri. The artificial transimission experiment verified that RLO could infect C. farreri but could not cause obvious histopathological change in a large scale. No evidence shows RLO in C. farreri has obvious pathogenicity and the conclusion is that RLO was not the main reason causing the high mortality of C. farreri in 2000 and 2001 along the Yellow Sea and Bohai Sea.更多还原