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猪囊尾蚴几种抗原氨基酸分析及其与抗原反应原性的相关性

Analysis of Amino Acids of Several Antigens and Its Relationship to Original Reaction of Antigens in Cysticercus Cellulosane

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【作者】 曹毅乔代蓉蒋彦杨滔

【Author】 CAO Yi 1, QIAO Dai rong 1, JIANG Yan 2, YANG Tao 2 ( 1National Laboratory of Grassland Biotechnology, Sichuan University, Chengdu 610064; 2Kaiya Biotechnology Corporation of Sichuan University, Chengdu 610064)

【机构】 四川大学草原生物工程国家实验室四川川大光耀生物工程有限公司 成都610064成都610064成都610064

【摘要】 通过对猪囊尾蚴虫体 (CWag)、囊液 (CFag)、头节 (CSag)、囊壁 (CBWag)、脲溶抗原 (UCWag)和成虫抗原(TSag)的水解氨基酸以及抗原反应原性的分析 ,确定了各氨基酸与抗原反应原性的关系。试验结果表明 ,几种抗原都含有 17种氨基酸 ,通过数据处理软件SPSS对各种抗原组成差异分析显示 ,各种抗原在天冬氨酸、谷氨酸、赖氨酸、组氨酸的组成上无显著差异 (P >0 .0 5 ) ,且CSag、CBWag之间在所有氨基酸组成上均差异不显著 ;而UCWag却与其它抗原在氨基酸组成上差异显著 (P <0 .0 5 )。UCWag的敏感性和特异性均较高 ,脯氨酸与抗原反应的敏感性呈正线性相关 ,谷氨酸与抗原反应的特异性呈正线性相关

【Abstract】 The amino acids in the six antigens in cysticercus cellulosane-cysticercuscyst fluid antigen (Cfag), C. Scolex antigen (CSag), C. Baldder wall antigen (CEWag), C. Worm antigen (CWag), Urea C. Worn antigen (UCWag) and T. Sotium adult (TSag) were determined quantitatively by automatic amino acid analyzer. The relationship of original reaction between amino acids and antigens was confirmed. The results showed that there were 17 amino acids among all of the antigens. There were no significant difference (P> 0.05 ) in composition of Asp, Glu, Lys and His of all antigens by data analysis software SPSS. There were also no significant difference (P>0.05) in the composition of all amino acids between Csag and CBWag. The composition of Thr, Ser, Tyr, Ved, Pro. in UCWag was significant difference (P<0.05) compared with other antigens. The sensitivity and peculiarity of UCWag were higher than that of the others. There existed significant positive correlation between Por. and sensitivity of antigen teaction, between Glu. and peculiarity of antigen reaction respectively.

【关键词】 猪囊尾蚴抗原氨基酸反应原性
【Key words】 CysticercuscysAntigenAmino acidsOriginal reaction
【基金】 四川省重点资助项目
  • 【文献出处】 中国农业科学 ,Scientia Agricultura Sinica , 编辑部邮箱 ,2002年07期
  • 【分类号】S852.7
  • 【下载频次】45
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