【摘要】 试验对超临界二氧化碳流体萃取番茄红素的条件 ,以及番茄红素对化学诱导转化细胞增殖、周期时相的影响进行了研究。结果表明 ,萃取番茄红素的最佳工作参数为 :萃取压力 2 5Mpa、萃取温度 5 0℃、萃取时间 3h。番茄红素有抑制细胞生长及细胞增殖的作用 ,并能明显改变细胞周期时相百分比 ,G1期百分比上升 ,S期 ,G2 +M期百分比下降 ,周期时相左移。番茄红素抑制细胞增殖 ,并使细胞周期时相左移可能是其抑制细胞恶性转化的重要原因更多还原

【Abstract】 The separation, extraction of lycopene and its effects on the proliferation and cell cycle of the chemical induced cells were investigated in order to research on its extraction method and the mechnism in inhibiting neoplastic transformation. The best extraction condition of lycopene with super critical carbon dioxide was under the pressure of 25Mpa, the temperature of 50℃and duration of 3.0h. Lycopene could inhibit cell growth rate and cell proliferation significantly, while increase the cell number of G 1 phase and decrease the cell number of S phase and G 2+M phase. The potency of the effects of lycopene on cell cycle might be one of the important reasons for inhibiting neoplastic transformation.更多还原