【摘要】 目的 :通过HCV结构区DNA疫苗直接免疫小鼠 ,诱发其体内特异性细胞介导的免疫反应的研究 ,为HCVDNA疫苗的研制打下基础。方法 :用重组的pBK CMV质粒体外转染小鼠骨髓瘤细胞SP2 0 ,建立了能体外表达HCV结构区蛋白的SP2 0D细胞系 ,分别用SP2 0和SP2 0D细胞攻击用空质粒或pBK CMV质粒免疫过的Balb c小鼠 ,通过肿瘤形成、肿瘤重量、肿瘤组织淋巴细胞的浸润以及不同免疫组小鼠血清中IL 2和IFN γ含量来观察小鼠体内T淋巴细胞的活性。结果 :用SP2 0和SP2 0D攻击空质粒或pBK CMV质粒免疫过的小鼠 15d后 ,SP2 0D攻击的pBK CMV质粒免疫鼠肿瘤重量〔(0 9± 0 12 )g〕明显低于空白质粒免疫组和SP2 0接种组 (P <0 0 0 1) ,且该免疫组小鼠血清中IL 2和IFN γ的浓度明显高于其它免疫组 ;此外 ,用SP2 0D攻击的pBK CMV免疫鼠 ,肿瘤病理切片中可见明显的T淋巴细胞浸润。结论 :重组的HCV结构区DNA疫苗 (pBK CMV)能诱导小鼠体内特异性T淋巴细胞反应 ,HCVDNA疫苗可能为临床疾病的治疗和预防提供一种新的途径更多还原

【Abstract】 Objective:To study the cell mediated immune repsonses in mice induced by direct plasmid pBK CMV immunization and pave a way for the further research of HCV DNA vaccine Methods:Used recombinant plasmid pBK CMV to transfect a syngeneic SP2/0 myeloma cells and established SP2/0D cell lines which can express HCV structural proteins DNA immunized mice were inoculated with native SP2/0 or SP2/0D cells respectively Activity of T lymphocytes was observed through tumor formation, tumor weight,T lymphocytes infiltration in tumor tissue and the level of IL 2,IFN γ in the serum of all immunized mice Results:Inoculated with SP2/0D or native SP2/0 15 days later, tumor weight in the SP2/0D inoculated mice immnunized with pBK CMV plasmid was remarkably reduced compared with mice immunized with blank plasmid,or inoculated with SP2/0 The level of IL 2 and IFN γ in the serum of pBK CMV immunized groups were higher than those of blank plasmid immunized mice In addition, the phenomena of T lymphocyte infiltration in tumor tissues were very apparent in the SP2/0D inoculated mice immnunized with pBK CMV plasmid Conclusion:The results indicate that recombinant plasmid pBK CMV can induce significant T lymphocytes activity in vivo by this technique, and HCV DNA immunization is a promising approach for vaccine development更多还原