【摘要】 脂肪氧合酶在塑料工业中的增塑剂制取上有较为广泛的应用前景 ,其次在青草芳香的自然风味工业上也有较为重要的作用。本文主要阐述了用硫酸铵二次盐析法从大豆低温浸出粕中提取大豆脂肪氧合酶的工艺。通过在不同的温度及pH环境下制取酶液 ,比较其活性 ,得出脂氧酶最佳提取条件为温度 2 0℃ ,pH4 .5。更多还原

【Abstract】 Lipoxygenase can catalyze the reaction producing hydroperoxides which are used as stabilizers-plasticisers for PVC.This project dealt with the method of using two steps salting out with (NH 4) 2SO 4 to get lipoxygenase from soybean flake extracted at low temperature.With different temperature and pH conditions tested,the optimal condition of 20℃, pH4.5 for the procedure was obtained.更多还原