【摘要】 目的 :建立大肠癌细胞中提取和测定 5-Fu含量的 HPL C方法 ,并观察药物浓度、时间及给药次数与大肠癌细胞内 5-Fu含量的关系。方法 :用 0 .1%氢氧化钠裂解大肠癌细胞后用乙醚-异丙醇 ( 8∶ 2 )提取细胞内 5-Fu,HPL C法测定大肠癌细胞内 5-Fu含量。分析条件 :Spherisorb C18柱 ,流动相 :0 .0 5m ol/L 磷酸盐缓冲液 ,紫外检测波长 :2 65nm。结果 :5-Fu进样量在 0 .0 0 0 52 6μg～ 0 .42 0 8μg之间呈良好线性关系 ,线性方程为 Y=3 154159X-40 8.89,r=0 .9997;5-Fu的最小检测限为 5.2 6ng;5-Fu不同加入量的的平均回收率分别为 82 .15%和 87.50 % ;重复性试验 RSD=6.72 %。结论 :用 HPL C法测定大肠癌细胞中 5-Fu含量的方法是可行的 ;大肠癌细胞对 5-Fu的摄取可能存在“饱和现象”,当给药浓度达到一定量 ( 5μg/m L)时 ,癌细胞中 5-Fu含量最高。更多还原

【Abstract】 Objective:To set up a method for extracting and measuring the content of 5 fluorouracil(5 Fu)in colorectal neoplasma cell(colo 205 cell) and to explore the relationship of drug concentration, 5 Fu exposure time and drug treating times with the content of 5 Fu in colo 205 cell.Methods:5 Fu was measured by reversed phase high performance liquid chromatography(RP HPLC) using a Spherisorb C 18 column and 0.05 mol·L -1 phosphate buffered saline(PBS) as mobile phase. 5 Fu was detected by UV absorbance at 265 nm.Results:The method proved to have linear relationship in the range of 0.000 526 μg～0.4 208 μg of the sampled quantum of 5 Fu. The linear equation was Y=3 154 159X-408.89, r =0.9 997. The lowest detection limit of 5 Fu was 5.26 ng. The average recovery was 82.15 %and 87.50 % respectively when different 5 Fu quantum was added and RSD was 6.72 %.Conclusion:The result showed that this method is feasible,efficient and operationable. There may be a satuation phenomenon at the absorption of colo 205 cell to 5 Fu. The level of 5 Fu in colo 205 cell would be at a top when the drug concentration was at certain quantum (5 μg/mL).更多还原