【摘要】 目的　研究大鼠局灶性脑缺血再灌注后神经细胞 bcl- 2、bax和 p53m RNA表达的变化规律。方法　利用大鼠大脑中动脉缺血再灌注模型 ,原位杂交技术分别观察缺血 1 .5 h再灌注后 2 h、 6 h、 1 2 h、 1 d、 2 d、 3 d、 7d和 1 4 d不同时间点神经细胞 bcl- 2、 bax和 p53 m RNA的表达。结果　脑缺血再灌注 2 h在缺血周围区 bcl- 2 m RNA开始表达 ,1 d达高峰 ,之后逐渐减弱 ,再灌注 1 4 d接近假手术组水平 ;bax m RNA与 p53 m RNA均于缺血再灌注 6 h出现 ,bax m RNA于 1 d达高峰 ,3 d降至假手术组水平 ,p53 m RNA于 1～ 2 d达高峰 ,7d降至假手术组水平。结论　脑缺血再灌注后 bcl- 2、 bax和 p53m RNA的表达与缺血性损伤有一定关系 ,参与了神经细胞凋亡的调节更多还原

【Abstract】 Objectives To study the expression and variation of bcl 2, bax and p53 mRNA in neurons after focal cerebral ischemia/reperfusion in rats. Methods Using rats model with middle cerebral artery occluded and adopting the technique of in situ hybridization, we detected the expression of bcl 2, bax and p53 mRNA on different time spots(2,6,12 hours and 1,2,3,7,14 days) after reperfusion of focal cerebral ischemia in rats.Results In the peripheral area of the infarction, bcl 2 mRNA positive neurons were observed 2h after reperfusion, peaked at 1d, decreased to normal level after 14 d; bax and p53 mRNA were detected at 6h, they reached their peak at 1d and 2d and decreased to control level on the 3rd d and 7th d after reperfusion respectively.Conclusions The expression of bcl 2, bax and p53 mRNA in neurons cerebral ischemia/reperfusion was related to ischemic injury and may play a regular role in neuronal apoptosis.更多还原