【摘要】 目的:研究ICA,PJM逆转肿瘤细胞恶性表型,提高免疫效应细胞识别和杀伤的作用和机制。方法:MTT法检测ICA,PJM对PG细胞增殖的影响以及对CD3AK杀伤敏感性的影响;RT-PCR法检测ICA,PJM对bcl-2和c-fos基因mRNA表达水平的影响;流式细胞术检测细胞表面HLA-A,B,C抗原表达的变化以及c-fos,bcl-2蛋白表达水平的变化。结果:ICA,PJM对PG细胞有明显的增殖抑制作用,可以提高细胞表面HLA-A,B,C分子的表达和c-fos蛋白的表达,抑制bcl-2基因的表达,提高CD3AK细胞对PG细胞的杀伤活性。结论:ICA,PJM可以逆转肿瘤恶性表型,提高免疫效应细胞对肿瘤细胞的识别和杀伤。更多还原

【Abstract】 Objective:To explore the mechanism and reversion of malignant phenotypes in a highly metastatic human lung tumor cell line. Methods: Suppression of 1C A and PJM on PG cell line and the effects of PJM and 1C A on PG cells sensitivity to lysis by CD3AK effectors cell was studied by MTT assay; the expression of bcl-2 and c-fos was studied by RT-PCR and flow cytometry, the expression of HLA-A, B, C in PG cells was examined by flow cytometry. Results:ICA and PJM inhibited the growth of PG obviously. ICA and PJM could significantly enhance the expression of HLA-ABC antigen and c-fos gene. ICA and PJM decreased the expression of bcl-2 and enhanced the the susceptibility of PG cells to lysis by CD3AK cells. Conclusion:ICA and PJM can reverse the malignant phenotypes of PG cells, enhance the recognition and killing effect of T cells.更多还原