【摘要】 目的 :探讨三氧化二砷 (As2 O3)对H2 2肝癌荷瘤小鼠免疫功能的影响及实体瘤生长情况。方法 :利用流式细胞仪及改进的MTT还原法分别对正常小鼠、荷瘤小鼠对照组、荷瘤小鼠As2 O3治疗组进行T细胞亚群及NK细胞活性 (NKCA)的测定 ,并比较各组瘤重 ,计算瘤重抑制率。结果 :肝癌小鼠荷瘤对照组与正常组比较 ,CD3 +,CD4+,CD4+ CD8+及NKCA均明显减少 (P <0 .0 1) ;低剂量及高剂量As2 O3治疗组与荷瘤对照组比较 ,CD3 +,CD4+,CD4+ CD8+及NKCA均明显升高 (P <0 .0 5 ) ,CD8+无明显变化 ;而高剂量As2 O3治疗组与低剂量As2 O3治疗组比较 ,CD4+,NKCA明显升高 (P <0 .0 5 ) ,CD3 +,CD4+ CD8+无明显变化。治疗组瘤重抑制率分别为 3 9.12 % ,45 .74% ,病理观察可见间质内大量炎症细胞浸润。结论 :AS2 O3能抑制实体肿瘤的生长 ,提高肝癌小鼠的免疫功能 ,这是一值得继续研究的临床课题更多还原

【Abstract】 Objective: To explore the immune effects and the solid tumor growth of arsenic trioxide on experimental H22 Hepatoma bearing mice.Methods: T lymphocyte subsets and NKcells activity were assessed by flow cytometry and improved MTT assay. Calculate the tumor inhibitory rates. Results: The percentage of CD3 + cells and CD4 + cells, the ratio of CD4 + cells to CD8 + cells, the activity of NK cells in the tumor control group were markedly lower ( P <0.01) than those in the healthy control group. However, they were significantly higher in the tumor treatment than in the tumor control group. There was a higher percentage of CD4 + and NKCA in arsenic trioxide treatment group at high dosage than that at low dosage ( P <0.05). The tumor inhibitory rates were 39.12% and 45.74% separately. A lot of inflammatory cells could be seen under optic microscopy. Conclusion: Arsenic trioxide can obviously increase the immune function of H22 Hepatoma bearing mice and inhibit the growth of tumor body.更多还原