【摘要】 以螺旋霉素链霉菌SPM1 1 0 8为出发菌株 ,经波长为 2 54nm、功率 30W的紫外诱变处理 50s,筛选豆油耐性突变株 ,得到一株螺旋霉素高产菌株SPM2 89,其发酵效价较出发菌株提高了 2 2 5 %。以此为试验菌株 ,采用含豆油培养基进行发酵试验 ,结果表明 ,豆油的最佳加入量为 2 0 % ,加入时间以 0～ 1 2h为宜。采用上述含豆油培养基并补加前体正丙醇 ,发酵效价比不添加前体提高 2 1 %。此工艺应用于 50m3发酵罐工业生产 ,月平均发酵水平较原工艺提高 30 %～ 50 % ,产品质量稍有提高更多还原

【Abstract】 The spiramycin producing strain Streptomyces spiramyceticus SPM1 108 as the parent strain was treated with UV irradiation (254nm, 30W, 50s), the oil tolerant mutants was screened and a high titer strain SPM2 89 was obtained, its potency was increased by 22 5% over the parent strain SPM1 108. Result from the fermentative experiments on the strain SPM2 89, showed that the optimum proportion of soybean oil in the production medium was 2 0% and the optimal feeding time of soybean oil was at the initial stages (0～12h) of fermentation. Feeding of propyl alcohol as precursors in above medium, the productivity was increased by 21%. In 50m 3 fermenter, the productivity increased by 30%～50%, and the quality of product was also improved.更多还原