【摘要】 目的 :探讨促排卵周期卵巢对促性腺激素反应性与颗粒细胞促卵泡激素受体 (FSHR)表达的关系。方法 :根据取卵时卵泡发育数不同 ,将卵巢对促排卵药物的反应分为低反应型 (卵泡数≤ 3个 )、中反应型 (卵泡数 4～13个 )和高反应型 (卵泡数≥ 14个 ) ,分别为 11例、15例和 13例。采用逆转录聚合酶链反应 (RT- PCR)测定 3种反应类型的卵泡颗粒细胞 FSHR m RNA的表达量。结果 :低反应型 FSHR m RNA表达量显著低于中、高反应型 (相对积分值分别为 0 .5 4± 0 .0 7、0 .90± 0 .17和 1.2 0± 0 .4 5 ,P<0 .0 5 )。结论 :卵巢对促性腺激素的反应与颗粒细胞FSHR m RNA的表达量高低有关。更多还原

【Abstract】 Objective: To investigate the relationship between the ovarian response,induced by gonadotropin hormone, and levels of follicle stimulating hormone receptor (FSHR). Methods:The level of ovarian response was divided into three groups based on the number of follicles obtained during oocyte retrieval: poor responders (<4 follicles), normal responders (4～13) and high responders (>13 follicles). FSHR mRNA was measured using reverse transcription polymerase chain reaction (RT PCR). Results: The expression of FSHR mRNA in the poor responders was 0.54±0.07. This was much lower than that of the normal responders who were at 0.90±0.17, and the high responders who were at 1.20±0.45( P <0.05). Conclusion: Ovarian response induced by gonadotropin hormone stimulation is correlated with the level of FSHR mRNA in the granulosa cells.更多还原