【摘要】 目的　研究核转录因子 κB (NF κB)及其抑制因子IκB基因在自体移植静脉中的表达 ,及其在内膜增生中的作用。方法　Wistar大鼠 80只 ,建立自体移植静脉模型 ,术后随机分为 6、2 4h ,3、7d ,2、4、6、8周等 8组 ,于相应时点取材 ,进行病理形态学研究 ,半定量逆转录PCR检测NF κBp6 5mRNA和IκBβmRNA表达的变化 ,原位杂交方法检测NF κBp6 5mRNA表达 ,联合应用Western蛋白印迹和免疫组织化学方法检测p6 5、IκBα和IκBβ的蛋白质表达。 结果　移植静脉术后 6h即出现p6 5mRNA和IκBβmRNA表达增强 ,基因表达值分别为 16 %± 4 %和 31%± 9% ,与正常静脉比较差异有非常显著意义 (P <0 0 1) ;p6 5mRNA表达 3～ 7d达高峰 ,术后 3、7d的表达值分别为 37%± 12 %和34%± 10 % ,IκBβmRNA表达则于术后 1～ 2周达到高峰 ,表达值分别为 5 3%± 17%和 4 9%± 10 % ,与其余各时点比较差异有非常显著意义 (P <0 0 1)。p6 5蛋白质阳性表达 1周达高峰 32 %± 13% ,IκBα、IκBβ蛋白质术后 2周达高峰 ,阳性率分别为 35 %± 11%和 4 4 %± 13% (P <0 0 1)。IκBα、β蛋白质术后 6～ 2 4h表达量有所降低 ,为正常静脉的 1/3～ 1/2。结论　移植静脉中存在NF κB系统及其抑制因子IκB基因的活化。核转录因子κB可能更多还原

【Abstract】 Objective To investigate the gene expression of nuclear transcriptional factor κB(NF κB) p65 and its inhibiting factor I κB in autogenous vein graft. Methods The right common jugular vein was transplanted to infrarenal abodminal aorta by microsurgical technique among 80 Wistar rats so as to establish an autogenous vein graft model. Ten vein graft samples were harvested 6 hours, 24 hours, 3 days, 7 days, 2 weeks, 4 weeks, 6 weeks, and 8 weeks after surgery. NFκB p65 mRNA and IκBβ mRNA were measured by reverse transcription PCR and in situ hybridization. Western blotting and immunohistochemistry were used to detect the protein expression of NFκBp65 and IκB. Results The expressions of NFκB p65 mRNA and IκBβ mRNA 6 hours after the surgery were 16%±4% and 31%±9% respectively ( P <0.01 vs. control vein). The expression of NFκB p65 mRNA reached in peak during the period 3 days to 7 days after the surgery (37%±12% and 34%±10% respectively, P <0.01 vs. other teams), however, the IκBβ mRNA expression reached its peak during 1 to 2 weeks after the surgery (53%±17% and 49%±10% respectively, P <0.01 vs. other teams). The expressions of NFκBp65 mRNA and IκBβ mRNA recovered to their baseline values 6 weeks after surgery. The expression of p65 protein reached its peak 1 week after the surgery (32%±13%) and then decreased gradually. The expressions of IκBα and IκBβ decreased to 1/3 to 1/2 of the normal vein 6 hours to 24 hours after the surgery and then increased to 5 times that of the control vein 2 weeks after surgery (35%±11% and 44%±13% respectively). Conclusion The NF κB/IκB system is activated in autogenous vein graft. The NFκB may become a new target for the prevention and therapy of intimal hyperplasia and stenosis after vein graft.更多还原