【摘要】 目的　研究 p16、p15基因缺失状态与喉癌发生、发展的关系。方法　提取喉癌新鲜组织中基因组 DNA,采用聚合酶链反应技术 ,分别对 80例喉癌进行 p16基因第 2外显子 (p16 E2 )和 6 7例喉癌进行 p15基因第 2外显子 (p15 E2 )纯合性缺失研究。结果　检测 p16 E2纯合性缺失率为 12 .5 % (10 / 80 ) ,p15 E2纯合性缺失率为 11.94% (8/ 6 7) ,p16 E2、p15 E2共同缺失率为 5 .97% (4 / 6 7)。结论　 p16 E2和 p15 E2纯合缺失以及二者共同缺失与喉癌发生有相关性 ,可能在喉癌发生及恶性进展中起一定作用。更多还原

【Abstract】 Objective To assess the relationship of homozygous deletion status of p16 (MTS1/ INK4a/ CDKN2A), p15(MTS2/INK4b/CDKN2B) genes and laryngeal squamous cell carcinoma(LSCC) progression. Methods DNA was extracted from fresh tumors. Homozygous deletion of p16 exon 2(p16E2) in 80 cases of LSCC and p15 exon 2(p15E2) in 67 cases of LSCC were detected by the polymerase chain reaction technique. Results The p16E2 deletion rate in 80 cases was 12.5%(10/80); the p15E2 deletion rate in 67 cases was 11.94%(8/67); the p16E2 and p15E2 codeletion rate in 67 cases was 5.97%(4/67). Conclusion Homozygous deletion of p16E2 and p15E2 is related with LSCC oncogenesis, and it may play a role to some extent in LSCC malignant progression.更多还原