【摘要】 目的　探索供者TNFα - 30 8(G A)基因型在Ⅲ Ⅳ度急性移植物抗宿主病 (aGVHD)中的作用。方法　排除可能影响aGVHD发生的因素 ,根据组间均衡原则 ,选择 2 1例Ⅲ Ⅳ度aGVHD患者及其同胞供者为研究对象 ,2 8例 0 Ⅰ度aGVHD患者及其同胞供者为对照组 ,通过变性高效液相色谱(DHPLC)结合DNA测序检测TNFα - 30 8基因型 ,用ELISA方法检测患者血清中TNFα含量。结果　通过DHPLC检测 ,2 1例Ⅲ Ⅳ度aGVHD患者的同胞供者中 13例 (6 2 % )呈现单峰 ,经DNA测序验证为TNFα - 30 8(G G) ;8例 (38% )呈现双峰 ,经测序验证为TNFα - 30 8(G A)。 2 8例 0 Ⅰ度aGVHD患者的同胞供者中 2 7例 (96 .4 % )呈现单峰 ,经测序验证为TNFα - 30 8(G G) ;1例 (3.6 % )呈现双峰 ,经测序验证为TNFα - 30 8(G A)。TNFα- 30 8(G A)在上述两组患者的同胞供者中的阳性率经 χ2 检验 ,P <0 .0 5 ,提示两组差异有显著性。通过ELISA检测的Ⅲ Ⅳ度aGVHD患者中 ,TNFα在TNFα - 30 8(G A)患者中的含量明显高于TNFα - 30 8(G G)的患者 (P <0 .0 5 )。在TNFα - 30 8(G G)的患者中 ,TNFα在Ⅲ Ⅳ度aGVHD患者中的含量明显高于 0 Ⅰ度aGVHD的患者 (P <0 .0 1)。结论　TNFα - 30 8(G A)基因型与体内TNFα含量及Ⅲ Ⅳ度aGVHD的易感性关?更多还原

【Abstract】 Objective To study the relationship between donor TNFα-308(G/A) genotype and recipient acute graft versus host disease (aGVHD). Methods TNFα-308(G/A)and TNFα-308(G/G) genotypes were analyzed by DHPLC and DNA sequence in twenty one degree Ⅲ/Ⅳ(Ⅲ/Ⅳ) aGVHD patients, and twenty eight degree 0/Ⅰ(0/Ⅰ) aGVHD patients.Serum TNFα levels were determined by ELISA. Results The frequency of TNFα-308(G/A)genotype was significantly higher in Ⅲ/Ⅳ aGVHD patients than in 0/Ⅰ aGVHD patients (8/21 vs 1/28)(P<0.05);and so did for higher serum TNFα levels(P<0.05). TNFα levels were higher in Ⅲ/Ⅳ aGVHD patients than in 0/Ⅰ aGVHD patients in TNFα-308(G/G) patients (P<0.01). Conclusion TNFα -308 (G/A) is positively related to serum TNFα levels and most likely contributes to high risk for Ⅲ/Ⅳ aGVHD.更多还原