【摘要】 水通道蛋白是一组水选择通道的分子家族 ,它们都有增加浆膜面水通透力的功能 ,因而可以提供快速液体转运的通路。本实验的目的是确定AQP1及AQP5是否在大鼠肺泡毛细血管膜 (呼吸膜 )表达 ,进而研究内毒素诱导的急性肺损伤 (ALI)大鼠AQP1和AQP5的表达调节及激素干预的作用。方法　采用亲和的抗人AQP1和AQP5抗体 ,应用免疫组化及免疫电镜的方法研究AQP1及AQP5在呼吸膜的分布。选用肺泡内灌注脂多糖 (LPS)制作大鼠ALI动物模型 ,研究ALI时呼吸膜AQP1及AQP5的变化。结果　免疫染色显示AQP1主要表达于正常肺组织的微血管内皮 ,而AQP5主要表达于肺泡I型上皮细胞。免疫组化分析进一步表明LPS灌注后 4h - 48hAQP1及AQP5在呼吸膜的表达均下降 ;AQP1蛋白于LPS灌注后 2 4h及激素干预后有部分恢复 (P <0 0 5 ) ,而AQP5无这种恢复现象。结论　本研究显示ALI时AQP1及AQP5在呼吸膜的表达减少 ,提示ALI时AQP1和AQP5的下降表达可能与其液体转运的异常有关。更多还原

【Abstract】 Objective To determine if aquaporin1 (AQP1) and aquaporin5 (AQP5) are expressed in the alveolar capillary membrane in rats Moreover, to investigate the alteration of AQP1 and AQP5 in acute injured lungs Methods The distribution of AQP1 and AQP5 in alveolar capillary membrane were investigated by immunohistochemistry and immunoelectron microscopy with affinity purified antibodies to human AQP1 and AQP5 To study the possibility that alveolar capillary membrane AQP1 and AQP5 undergo altered regulation, we established a rat model using alveolar instillation of lipopolysaccharide (LPS) Results Immunolabelling showed AQP1 was stained primarily in the microvascular endothelia of normal lungs, while AQP5 was expressed in type Ⅰ pneumocytes Immunohistochemical analysis showed a significant decrease in the expression of AQP1 and AQP5 in injured lungs at 4h-48h after LPS instillation AQP1 protein was resumed partly at 24h after LPS instillation and steroid administration, whereas AQP5 was unchanged Conclusion The decreased expressions of AQP1 and AQP5 in injured lungs suggest that both of them may play a role in abnormal fluid transportation更多还原