【摘要】 目的　研究人乳腺癌异种移植的自发转移、遗传稳定性和微转移的检测方法。方法　将人乳腺癌新鲜组织移植于裸鼠并连续传代 ,移植瘤细胞体外培养、再移植入裸鼠并在三个微卫星位点分析人乳腺癌、裸鼠移植瘤及其转移灶的微卫星DNA。结果　原位移植的成瘤率为 88 6 %(31/ 35 )、转移率为 4 1 9%(13/ 31)。移植瘤细胞体外培养成功 ,其再移植裸鼠成瘤率和转移率均达 10 0 %,裸鼠移植瘤及其转移灶在三个位点与人乳腺癌具有相同的微卫星DNA。结论　完整组织原位移植法可提高人乳腺癌异种移植的成功率和转移率。异种移植瘤及其传代肿瘤保持遗传稳定性。微卫星DNA鉴定法可以检测裸鼠模型的微转移。更多还原

【Abstract】 Objective To investigate spontaneous metastasis, micrometastasis and genetic stability in human breast carcinoma xenografts in nude mice.Methods Intact tissue from surgical specimens from breast carcinoma patients was xenografted into nude mice and transplanted from generation to generation. Cells from the xenografts were cultured in vitro and retransplanted into nude mice. Microsatellite DNA in the genome of human breast carcinomas, xenotransplanted tumors and metastases in nude mice were analyzed at three microsatellite loci.Results The tumorigenicity of orthotopic xenotransplantation was 88.6% (31/35), with a metastatic rate of 41.9% (13/31). Cells from xenotransplants were successfully cultured in vitro. The taking rate of retransplantation into nude mice and the spontaneous lung metastasis rate were both 100% (10/10). Microsatellite DNA sequences in the genome of xenotransplanted tumors and metastases in nude mice were identical with that of the original human breast carcinoma at three microsatellite loci.Conclusions Tumorigenicity and metastatic potential can be improved in human breast carcinoma xenografts using intact fresh tumor tissue and orthotopic grafts. Xenotransplanted tumors and tumors after serial passage maintained the genetic stability. The detection of microsatellite DNA may identify micrometastases in a nude mouse model.更多还原