【摘要】 目的　了解甲型流感病毒H9N2亚型毒株在深圳地区鸡群和人群中的分布。方法　采用常规的鸡胚双腔法来分离病毒。抗体测定 ,采用红细胞凝集抑制 (HI)试验和中和试验测定法。结果　从深圳地区农贸市场鸡群中分离到 2 7株H9N2亚型流感病毒 ,但未能从人群中分离到H9N2病毒。约有 2 6 %人血清中检测到H9亚型毒株的抗体 ,(HI滴度≥ 2 0 ) ,同时还发现抗体阳性率和几何均数随人群年龄增长而增高 ,同时与职业有关。然而 ,在鸡群中H9毒株的抗体阳性率仅为 7%。结论　禽H9N2毒株不仅能感染人 ,而且在深圳地区人群和禽类中较为广泛的分布。人H9N2很大可能来源于鸡的H9N2毒株。更多还原

【Abstract】 Objective To understand the distribution of influenza A H9N2 virus in chickens and men in Shenzhen area. Methods Virus isolation was performed in embryonated hen’s eggs with routine method. The antibody to H9N2 virus was detected with micro hemagglutination inhibition (HI) test, then the results were checked by using the neutralization assay in MDCK cells. Results Totally 27 strains of influenza A H9N2 virus were isolated from chickens in farm markets in Shenzhen, whereas no H9N2 virus was isolated from men. Approximately 26% of human sera with the HI titers ≥20 to H9N2 virus were detected.However only 7% of chicken sera with the HI titers ≥20 to H9N2 virus were detected.Meanwhile the HI titer and (MGT) of antibody to H9N2 virus in human sera increased with age.It was also found that there was a close relationship between HI antibody titer to H9N2 virus in human sera and occupation. Conclusion The distribution of influenza A H9N2 virus in chicken and men in Shenzhen was rather wide. The human H9N2 virus infection probably derived from chicken H9N2 virus.更多还原