【摘要】 目的　探讨临床诊断为非甲～戊型肝炎患者的病原学。方法　采用巢式PCR(nPCR)检测HBV、TTV、B19、和SENVDNA ;用逆转录巢式PCR(RT nPCR)检测HGV和HCVRNA。结果　 6 0例临床诊断为非甲～戊型肝炎患者中 ,单独HBVDNA阳性 30例 (阳性率为 5 0 0 % ) ,HBV和TTVDNA阳性10例 (16 7% ) ,HBV和B19DNA阳性 6例 (10 0 % ) ,HCVRNA、HBV和SENVDNA阳性 1例 (1 7% ) ,单独HCVRNA阳性 1例 (1 7% ) ,HCVRNA和B19DNA阳性 1例 (1 7% ) ,HGVRNA无一例阳性 ,单独B19DNA阳性 2例 (3 3% ) ,单独TTVDNA阳性 1例 (1 7% ) ,另 8例 (13 3% )上述病毒核酸均为阴性。HBV合并感染TTV或B19对其血清学生化指标无影响。结论　HBV是临床诊断为非甲～戊型肝炎的主要病原 ,HGV、TTV、B19和SENV与非甲～戊型肝炎无关更多还原

【Abstract】 Objective To study etiology of clinically diagnosed non A-E hepatitis.Methods HBV,TTV,human parvovirus B19,SENV DNA were detected by nested polymerase chain reactions(nPCR),while HGV,HCV RNA were tested by reverse transcription nested polymerase chain reactions (RT|nPCR).Results Of 60 patients with clinically diagnosed non A|E hepatitis,30(50.0%) were HBV DNA positive alone,10(16.7%) HBV and TTV DNA positive,6(10.0%)HBV and B19 DNA psoitive;1(1.7%)HBV,SENV DNA and HCV RNA positive,1(1.7%)HCV RNA positive alone,1(1.7%) HCV RNA and B19 DNA positive,2(3.3%)B19 DNA positive alone,1(1.7%) TTV DNA positive alone,and the remaining 8(13.3%) negative for all viruses.All the 60 patients were HGV RNA negative.There were no differences in serum biochemical markers of hepatitis B patients with or without TTV or B19 virus infection.Conclusion HBV is a major etiologic agent for the clinically diagnosed non A|E hepatitis.HGV,TTV,B19 and SEBV may not be associated with non A|E hepatitis.更多还原