【摘要】 目的　探讨胚胎睾丸异位移植到成年大鼠体内是否能生长发育 ,并具内分泌功能。方法　将胚胎睾丸分别移植至成年雄性Wistar大鼠颈部皮下和肾被膜下 (移植实验组 ) ,并设实验对照组 (摘除睾丸 )和正常对照组 ,移植实验组于移植后第 5d和 10d、2 5～ 35d和 35d以上取出移植物 ,同时断头取血 ,经离心取上清液。常规染色观察移植物的生长发育情况 ;细胞化学染色、免疫组织化学染色和放射免疫法检测移植物功能状态。结果　移植的胚胎睾丸在成年大鼠体内可生长发育至成年状 ;睾丸间质 3 β羟甾脱氢酶和油红O细胞化学染色呈阳性反应 ,睾丸间质细胞雄性激素及其受体、孕酮及其受体呈不同程度的阳性反应 ;血清睾酮、孕酮、卵泡刺激素和黄体生成激素接近正常值。结论　胚胎睾丸异位移植到成年大鼠体内后可正常发育 ,并具内分泌功能更多还原

【Abstract】 Objectives To investigate the status growth and development and function of fetal testis after transplanting into adult male rats.Methods Wistar adult male rats were divided into control group (normal and dividing testis) and transplantation experimental group (fetal testis grafted subcutaneously in neck and under renal capsule). On day 5, 10, 25-35 and over 35 after transplantation, grafts were removed. At the same time, blood was collected by decapitation and supernatant obtained by centrifugation. The growth and development of implant were studied histologically. The function of implant was studied by histochemistry, immunohistochemistry and radioimmunoassay.Results The fetal testis could grow and develop after being transplanted into adult rats. 3-βHSDH, oil red O and A, AR, P, PR were positive in interstitial cells. T, P, FSH and LH of serum showed increases and were near normal levels.Conclusions The fetal testis after transplanting can grow and develop and has the function of secreting steroid hormones.更多还原