【摘要】 目的　探讨 2型糖尿病患者皮下及大网膜脂肪组织脂联素 (adiponectin)表达水平及与血脂联素、体重指数、腰臀比 (WHR)、胰岛素敏感性的相关关系。方法　用实时荧光定量RT PCR检测 2型糖尿病患者和非糖尿病患者皮下及大网膜脂肪组织脂联素mRNA的表达水平 ,用ELISA方法测定血浆脂联素水平。结果　 2型糖尿病患者大网膜脂肪组织脂联素mRNA表达水平较非糖尿病组显著下降 (P <0 .0 5 ) ;糖尿病组与非糖尿病组的血浆脂联素水平差异无显著性 ;糖尿病组大网膜脂肪组织脂联素mRNA表达与WHR成负相关 (r=- 0 .5 1,P <0 .0 5 )。糖尿病组血浆脂联素水平与大网膜脂肪组织脂联素mRNA的表达成正相关 (r=0 .5 7,P <0 .0 1)。结论　 2型糖尿病患者大网膜脂肪组织脂联素mRNA表达显著降低。内脏脂肪组织脂联素mRNA的表达水平可以作为胰岛素抵抗的重要参数。更多还原

【Abstract】 Objective To study the adiponectin mRNA expression in subcutaneous and omental adipose tissues of type 2 diabetes and their relation with blood adiponectin, body weight index, waist hip ratio and insulin sensitivity. Methods The adiponectin mRNA expression level in adipose tissue of 22 cases with type 2 diabetes and 26 cases of non diabetes controls was assayed by real time fluorescence quantitative RT PCR. The level of plasma adiponectin was measured with ELISA kit. Results The adiponectin mRNA expression in omental adipose tissue was significantly decreased in patients with type 2 diabetes than that in non diabetes controls (P<0.05). The expression level of adiponectin mRNA in diabetic omental adipose was negatively correlated with waist hip ratio. No difference of plasma adiponectin level was found between two groups. Plasma adiponectin level was positively correlated with the mRNA level of omental adipose tissue in diabetes group. Conclusion Type 2 diabetic patients show lower transcription of adiponectin mRNA in omental adipose tissue than that of non diabetic control. The level of adiponectin mRNA in visceral adipose tissue seems to be an important parameter of insulin resistance.更多还原