【摘要】 目的　探讨海风藤新木脂素类成分对缺血鼠脑细胞间粘附分子 1(ICAM 1)及其mRNA表达的影响。方法　建立大鼠大脑中动脉闭塞 /再灌注模型 ,应用RT PCR及免疫组织化学的方法 ,观察海风藤新木脂素复合物以及银杏内脂对缺血鼠脑ICAM 1及其mRNA表达的影响。结果　免疫组织化学染色显示ICAM 1在假手术组鼠脑呈低表达 ;缺血 90min表达上调 ;缺血 90min再灌 2 4h后ICAM 1表达较前差异有非常显著性 (P <0 .0 1) ;而海风藤新木脂素类成分以及银杏内脂治疗组于相同时限鼠脑ICAM 1表达与单纯缺血、缺血再灌注组比较显著下调(P <0 .0 1)。假手术组鼠脑ICAM 1mRNA呈低表达 ;缺血 90min/再灌 2 4h其表达水平较对照组显著上调 (P <0 .0 1) ;药物治疗组于相同时限ICAM 1mRNA的表达显著下调 (P <0 .0 1)。结论　海风藤新木脂素类成分以及海风藤酮能明显降低缺血脑组织ICAM 1及其mRNA的表达 ,从而减轻脑缺血后炎性病理损害 ,达到脑保护作用更多还原

【Abstract】 Objective To investigate the effects of Piper Futokadsura Neoligans on intercellular adhesion molecule 1 (ICAM 1) and ICAM 1 mRNA expression in brain following focal cerebral ischemia. Methods The rat models of middle cerebral artery occlusion (MCAO) was established with the suture method, and ICAM 1 protein and ICAM 1 mRNA were measured in rat brain in the ischemic group and that in the treatment groups treated with Piper Futokadsura Neoligans by using immunohistochemistry and RT PCR techniques. Results By down regulating the expression of ICAM 1 and ICAM 1 mRNA and alleviating inflammation in the cerebral ischemic region, Piper Futokadsura Neoligans exerted a potent protective effect in cerebral ischemia and reperfusion. Conclusion The results suggested that platelet activating factor receptor antagonist Piper Futokadsura Neoligans had a potent effect in alleviating the impairments caused by cerebral ischemia and reperfusion.更多还原