【摘要】 目的　探讨BCL 2及P5 3mt蛋白筛检砷中毒致皮肤癌的价值。方法　BCL 2及P5 3mt蛋白的检测用免疫组化染色法 ;两指标阳性率的分析用 χ2 检验 ;分析评价筛检价值的有关指标。结果　无论是P5 3mt还是BCL 2 ,癌变组的阳性率均高于非癌变组 ,差异均有统计学意义 (P5 3mt ,P <0 .0 1;BCL 2 ,P <0 .0 5 ) ,其OR值分别为 14 .2 2 (2 .93～ 6 8.97)和 8.76 (1.0 7～ 71.5 1)。P5 3mt的约登指数为 0 .5 2 9,明显高于BCL 2的 0 .2 84。串联试验可提高筛检价值 ,并联试验则降低了筛检价值。结论　P5 3mt与BCL 2均可用于筛检砷中毒所致皮肤癌 ,将两指标串联可提高筛检价值。更多还原

【Abstract】 Objective To discuss the value of P53mt and BCL 2 proteins in screening skin carcinoma due to arseniasis. Methods P53mt and BCL 2 proteins were detected by immunohistochemical staining. χ 2 test was used to analyze the difference of positive rate between two groups. Screening value of the two biomarkers was also evaluated through the analysis of relative indexes. Results Positive percentages of P53mt and BCL 2 in carcinoma group were 88.89 % and 94.44 % respectively, both were higher than those of 36.0 % and 66.0 % in non carcinoma group (for P53mt, P < 0.01 ; for BCL 2, P < 0.05 ). ORs of P53mt and BCL 2 were 14.22 ( 2.93 68.97 ) and 8.76 ( 1.07 71.51 ), respectively. Youden’s Index and specificity of P53mt were 0.529 and 64.0 %, which were much higher than those of BCL 2. Serial tests improved the value of screening with Youden’s Index 0.569 , but parallel test lowered it to 0.244 . Conclusions P53mt and BCL 2 were practical biomarkers to screen skin carcinoma due to arseniasis, and the former was better than the latter. The value of screening can be improved by a series of tests.更多还原