【摘要】 目的　运用异源双链泳动分析法 (HMA )进行人类免疫缺陷病毒 1(humanimmunodeficiencyvirus 1,HIV 1)基因亚型分析 ,了解福建省HIV 1亚型的流行情况。方法　收集细胞培养中HIV 1感染的外周血单个核细胞 (PBMC) ,聚合酶链反应 (PCR)扩增HIV 1膜蛋白基因(env)区的核酸片段 ,与标准亚型的对照质粒的PCR产物进行杂交 ,形成异源双链二聚体 ,根据其在聚丙烯酰胺凝胶电泳中的泳动率确定其亚型 ,并进一步对标本进行序列测定和系统树分析。结果15份标本中 ,80 .0 0 %为E亚型 ,6 .6 7%为B亚型 ,2份不能确定其亚型。比较异源双链泳动法和序列测定法的结果 ,显示两种分析方法对HIV 1病毒基因分型有较高的一致性 ,其亚型一致率为86 .6 7%。结论　福建省流行的HIV 1毒株以E亚型为主。亚型分析法具有快速、简便、经济和高特异性的特点 ,可用作对HIV流行毒株进行长期监测的重要手段。更多还原

【Abstract】 Objective Using heteroduplex mobility assay(HMA) to subtype human immunodeficiency virus type 1(HIV 1) for the purpose of understanding HIV 1 subtype epidemic in Fujian province. Methods DNA fragments of HIV 1 env gene were amplified from peripheral blood mononuclear cell(PBMC) cocultures of HIV 1 infected individuals by nested polymerase chain reation(PCR). Heteroduplexs were formed through hybridizing PCR products from the samples and reference plasmid.According to the mobility of heteroduplexs in polyacrylamide gel electrophoresis, HIV 1 subtype from that sample was characterized and further confirmed by nucleotide sequencing analysis. Results Thirteen of 15( 86.67 %) samples were successfully subtyped by HMA,except 2 failures. Subtype E and B took up 80%(12/15) and 6.67 %(1/15) respectively. Results indicated a high concordance between HMA and nucleotide sequencing analysis and concordance rate was 86.67 %(13/15). Conclusions Subtype E appeared to be the major epidemic strain of HIV 1 in Fujian. HMA showed the characteristics of fastness, easiness, economie and with high specificity, and can be used in the survillence for the epidemic strain of HIV 1.更多还原