【摘要】 [目的]研究乳腺癌组织中金属蛋白酶组织抑制因子 （TIMP₁）的表达状况及其与Ⅳ型胶原 （ColⅣ ）的分布形式的关系 ,揭示乳腺癌的浸润转移机制 ,同时为可能应用新治疗方法提供理论依据。[方法]应用SP免疫组化方法检测95例乳腺癌组织和30例乳腺非癌组织中TIMP₁的表达情况和ColⅣ的分布形式。[结果] （1）在95例乳腺癌组织中 ,TIMP₁的阳性表达率为32.6%（31/95） ;ColⅣ分布形式中包裹型占14.7%（14/95）、崩解型占85.3%（81/95）。与对照组比较均有显著差异 （P<0.01）。（2）TIMP₁的表达与组织学分级有关 （Ⅰ级/Ⅲ级 ,P<0.05） ,级别越高 ,阳性率越高。（3）TIMP₁的表达与ColⅣ的分布形式相关（P<0.05） ,TIMP₁阳性者 ,则崩解型ColⅣ出现的机率小。[结论]乳腺癌TIMP₁的表达与肿瘤浸润或转移呈负相关 ,TIMP₁则通过下调MMP对ColⅣ的降解活性而抑制肿瘤细胞的侵袭 ,这为应用MMP抑制剂治疗乳腺癌提供客观依据更多还原

【Abstract】 To investigate the expression of TIMP₁ and the relation between TIMP₁ and the distribution form of ColⅣ in the tissue of breast carcinoma to disclose the mechanism of the tumor invasion and metastasis of breast carcinoma, and to find out the theory of a new therapy to breast carcinoma.Samples were obtained from 95 patients with breast carcinoma patients and 30 without breast carcinoma. The expression of TIMP₁ and the distribution form of ColⅣ were examined by immunohistochemical technique（SP）.（1） The positive rate of TIMP₁ expression in 95 cases of breast carcinoma were 32.6%（31/95）.The distribution form of encapsulated ColⅣ was 14.7%（14/95）,and the distribution form of decomposition ColⅣwas 85.3%（81/95）.There were significant different with those of the control group.（2）The expression of TIMP₁ was related to histologic grade（Ⅰ/Ⅲ,P<0.05）.（3）The expressions of TIMP₁ were related to the distribution form of ColⅣ （P<0.05）.[Conclusion]TIMP₁ are believed to suppress the invasive potential of breast carcinoma.It can provide the objective evi_dence for the inhibitor of MMP.更多还原