【摘要】 目的　研究低强度 2 4 5 0MHz微波是否增强丝裂霉素C(MMC)对人外周血淋巴细胞的遗传毒性。方法　采用彗星试验和胞质分裂阻断微核试验 (CBMN) ,在体外检测 2 4 5 0MHz微波 (5 .0mW/cm2 )与MMC诱发的DNA单链断裂及染色体损伤的情况。结果　微波辐射组外周血淋巴细胞的彗星长度 [男、女分别为 (2 9.1± 8.1)、(2 5 .9± 7.5 ) μm]与对照组 [男、女分别为 (2 6 .3± 6 .6 )、(2 4 .1±4 .3) μm]比较 ,差异无显著性 (P >0 .0 5 ) ;MMC各剂量组 (0 .0 12 5、0 .0 2 5 0、0 .0 5 0 0、0 .10 0 0 μg/ml)的彗星长度均长于对照组 ,差异有显著性 (P <0 .0 1) ,而且随着MMC剂量的增加 ,彗星长度增长 ;微波联合MMC(MW +MMC)各剂量组的彗星长度也随着MMC剂量增加而增长 ,且明显长于对照组 ,差异亦有显著性 (P <0 .0 1) ,当MMC≥ 0 .0 2 5 0 μg/ml时 ,微波与MMC可协同增加DNA单链断裂。微核试验结果表明 ,微波组的微核率与对照组的差异无显著性 (P >0 .0 5 )。MMC组和MW +MMC组在MMC≥0 .0 5 0 0 μg/ml时 ,其微核率与对照组比较 ,差异有显著性 (P <0 .0 5或P <0 .0 1)。MW +MMC组的微核率高于相应的MMC组 ,但差异无显著性 (P >0 .0 5 )。结论　低强度 2 4 5 0MHz微波辐射未能诱发人外周血淋巴细胞DNA和染色体损伤更多还原

【Abstract】 Objective To study that low intensity microwave whether or not enchances the genotoxic effects of mitomycin C(MMC) on human lymphocytes. Methods Single strand DNA breaks and chromosomal aberrations were measured by comet assay and cytokinesis blocked micronucleus(CBMN) test in vitro when human lymphocytes were exposed to 2 450 MHz microwave(5.0 mW/cm 2) alone and in combination with mitomycin C. Results In the comet assay,the average comet lengths of microwave group[(29.1±8.1)μm in male and (25.9±7.5)μm in female] were not significantly different from those of control groups [(26.3±6.6) and (24.1± 4.3) μm respectively]( P >0.05).The average comet lengths of MMC group(0.012 5,0.025 0,0.050 0,0.100 0 μg/ml)were significantly longer than those of control groups ( P <0.01) and were increased with the dose of MMC.The average comet lengths of microwave combined with MMC (MW+MMC) also were increased with the doses of MMC and were significantly longer than those of control groups ( P <0.01).When MMC was ≥ 0.025 0 μg/ml,microwave and MMC synergistically increased the single strand DNA breaks.In the micronucleus test,the average micronucleus rates of microwave groups were not higher than those of control groups ( P >0.05).The average micronucleus rates of MMC groups and MW+MMC groups were significantly higher than those of control groups ( P <0.01) when MMC was ≥0.050 0 μg/ml.The average micronucleus rates of MW+MMC groups seemed higher than those of corresponding MMC groups,however the difference was not significant ( P >0.05). Conclusion Low intensity(2 450 MHz) microwave did not induce DNA and chromosome damages on human lymphocytes,but enhanced the effects of DNA breaks induced by MMC.更多还原