【摘要】 目的　观察臭氧对家犬正常髓核组织的作用及其用于治疗腰椎间盘突出症的安全性。方法　家犬 5只 ,于透视下用 2 0GChiba针刺入犬腰椎间盘中心部 ,抽取 10ml臭氧 ,经穿刺针注入3ml,在椎间孔处将剩余气体注入椎旁组织。其中L 2～ 3和L 3～ 4注入臭氧浓度为 5 0 μg/ml,L4～ 5和L 5～ 6注入臭氧浓度为 30 μg/ml,以L6～ 7椎间隙作为正常对照。 3只犬注射臭氧 1次 ,分别在术后1周、1个月和 2个月后处死取标本 ,另外 2只犬注射臭氧 2次 ,间隔 1周 ,术后 1个月和 2个月处死取标本 ;将实验和正常对照标本中椎间盘髓核作大体、电镜下和光镜下观察 ,将相应水平的终板、脊髓、神经根和腰大肌作大体、光镜下观察。结果　术后实验动物均无严重的行为异常。术后 1个月和2个月 ,腰椎间盘髓核水分较正常明显减少而萎缩 ,胶原纤维明显增生。 2种不同浓度的臭氧对髓核影响无明显差别 ,注射 2次臭氧较注射 1次者髓核的萎缩程度更明显。 5处椎间盘水平的腰大肌标本内有少量肌纤维萎缩 ,16个终板呈轻到中度增厚。结论　本研究显示椎间盘内 2种浓度的臭氧注射安全性好、能使髓核缓慢萎缩 ,为该技术在临床的应用提供了实验依据。更多还原

【Abstract】 Objective To evaluate the influence of ozone on normal nucleus pulposus and the safety of intradiscal ozone injection for the treatment of herniated lumbar disc. Methods Ozone was injected into selected lumbar discs (3 ml) and the paraspinal space (7 ml) with 20G Chiba needle under fluoroscopy in five canines. The ozone concentration was 30 μg/ml and 50 μg/ml respectively. Two discs were selected for each concentration. Total 20 discs were injected. Three of the canines were given one time ozone injection and were sacrificed for pathology one week, one month and two months respectively after the procedure, and the other two canines were given two time ozone injection and were sacrificed one month and two months respectively after the procedure. The specimens including nucleus pulposus, end plate, spinal cord, nerve root, and greater psoas muscle were observed macroscopically and microscopically. Results No serious behavior abnormalities were observed in all animals. The atrophy of nucleus pulposus could be observed one month after ozone injection due to significant reduction of water and extensive proliferation of collagenous fiber. The influence on the atrophy of nucleus pulposus demonstrated no apparent difference between the selected two concentrations of ozone, but was more apparent with two time injection than that with one time injection. The end plates increased slightly or moderately in thickness in 16 samples and a few of fibers in greater psoas muscle suffered slight atrophy in 5 samples. Conclusion It is suggested that percutaneous intradiscal ozone injection is a safe method, and can cause gradual atrophy of nucleus pulposus. This study provides the evidence of the feasibility and value of this procedure′s application in clinics.更多还原