【摘要】 目的　探讨人类白细胞抗原 (HLA)系统及夫妻抗原共享率与妊娠高血压综合征 (妊高征 )的关系。方法　选择 5 7例妊高征患者及其丈夫、新生儿共 171例为研究组 ,46例同期正常孕妇及其丈夫、新生儿共 13 8例为对照组。采用快速盐析法提取孕妇及丈夫外周血 ,新生儿脐血白细胞DNA ,应用顺序特异性引物聚合酶链反应技术 (sequence specificprimerspolymerasechainreaction ,SSP PCR)进行等位基因检测。结果　单基因分析结果显示 ,研究组孕妇等位基因频率显著增高的有DRB1 0 3 0 6、DRB1 0 40 5、DRB1 0 3 15、DRB1 0 40 3、DRB1 13 0 4、DQB1 0 3 0 3 2 / 3 3、DQB1 0 5 0 2 ,其中DRB1 0 3 0 6、DRB1 0 40 5、DQB1 0 5 0 2等位基因风险处于最高值 (RR值分别为 9 76、6 3 4、4 2 0 ) ,基因频率显著高于对照组孕妇 ,差异有极显著性 (P <0 0 1) ;等位基因频率显著降低的有DRB1 0 40 7、DRB1 0 3 17、DQB1 0 5 0 3 1,与对照组比较 ,差异也有显著性 (P <0 0 5 )。研究组新生儿DQB1 0 5 0 2、DRB1 0 40 5等位基因频率显著高于对照组 ,差异有显著性 (P <0 0 5 ) ;DRB1 13 0 4、DQB1 0 5 0 3 1等位基因频率显著低于对照组 (P <0 0 5 )。两组夫妻抗原共享率比较 ,差异无显著性 (P >0 0 5 ) ,研究组母胎抗原共享率?更多还原

【Abstract】 Objective To study the relationship between human leucocyte antigen(HLA), parents′ sharing rate and the incidence of pregnancy induced hypertension (PIH), and investigate its immunopathogenetic roles to severe PIH Methods Newborn′s and their parents′ peripheral blood DNA was extracted and HLA phenotypes were studied by sequence specific primers polymerase chain reaction (SSP PCR) in 57 with PIH and 46 normal pregnancy Results The frequency of DRB1*0306, DRB1*0405, DRB1*0315, DRB1*0403, DRB1*1304, QB1*03032/33, DQB1*0502 in women with PIH was increased significantly and the frequency of DRB1*0407, DRB1*0317, DQB1*05031 were lower than controls And the risk to PIH of women with DRB1*0306, DRB1*0405, DQB1*0502 were higher than others ( RR : 9 76,6 34,4 20), and its gene frequency was higher than controls ( P <0 01) Frequcncy of DQB1*0502 , DRB1*0405 in PIH′s newborns were higher than controls ( P <0 05), but DRB1*1304, DQB1*05031 were lower than controls, with significant difference ( P <0 05); The gene frequency of fathers of the two groups weren′t different The HLA sharing rate of mothers or fathers of the two groups weren′t different But that of mother fetus was higher in PIH than controls Two locus alleles of PIH group constructed haplotype, and the DRB1*0315 DQB1*0502, DRB1*0405 DQB1*06012 antibody was positive with hypertension and protenuria ( P <0 05) Conclusion PIH is associated with HLA, and the over expression of HLA genes was a severe cause of PIH更多还原