【摘要】 目的　探讨BRCA2基因在遗传性卵巢癌组织中的突变 ,及临床意义。方法　采用BRCA2基因第 11外显子的一对引物 ,应用聚合酶链反应 单链构象多态性 (PCR SSCP)及非同位素DNA银染方法 ,检测 12例遗传性卵巢癌组织及 15例散发性卵巢癌组织中BRCA2基因第 11外显子6 174delT突变的情况。结果　 12例遗传性卵巢癌组织中有 2例检测到BRCA2基因第 11外显子6 174delT突变。 15例散发性卵巢癌组织中无一例BRCA2基因第 11外显子 6 174delT突变。结论　BRCA2基因突变可能与遗传性卵巢癌的发生有关更多还原

【Abstract】 Objective The aim of this study was to detect the mutation of BRCA2 gene in hereditary ovarian cancer tissues to probe the clinical significance of it Methods DNA was abstract from paraffin embeded tissues archived in phathology department A pair of primer located on 11 exon was used to detect the 6174 delT mutation,BRCA2 gene mutation in 12 hereditary ovarian cancer patients and 15 sporadic ovarian cancer patients were screened by polymerase chain reaction single strand conformation polymorphism analysis with DNA non isotopic silver staining methods Results Two of 12 hereditary ovarian cancer victims were found carrying mutation of BRCA2 gene The mutation type was 6174del T in the 11 exon of BRCA2 gene No mutation of BRCA2 gene on this site was found in 15 sporadic ovarian cancer patients Conclusion BRCA2 gene mutation was closely associated with the carcinogenesis and development of hereditary ovarian cancer, but had no relationship with sporadic ovarian cancer更多还原