【摘要】 目的　观察红霉素对离体人鼻息肉组织中嗜酸粒细胞凋亡的影响。方法　将鼻息肉组织 +红霉素 (10 -4mol/L)按外植块培养法培养 1、3、5d后作连续切片 ,应用末端脱氧核苷酸转移酶介导的脱氧三磷酸尿苷缺口末端标记法 (terminaldeoxynucleotidyltransferasemediateddUTPnickendlabeling,TUNEL法 )原位检测凋亡细胞 ,与相邻连续的HE染色片对位对照观察 ,鉴别凋亡细胞中的嗜酸粒细胞 ,并与不加红霉素培养的鼻息肉组织标本对照。结果　红霉素组培养 1、3和 5d的嗜酸粒细胞凋亡指数分别为 (31 77± 9 5 2 ) %、(32 6 7± 9 4 4 ) %和 (5 0 34± 8 78) % ,不加红霉素对照组嗜酸粒细胞凋亡指数分别为 (2 9 18± 7 31) %、(2 9 82± 12 0 3) %和 (42 2 5± 8 0 8) % ,培养 5d 2组间嗜酸粒细胞凋亡指数差异有显著性 (P <0 0 5 )。结论　红霉素对离体鼻息肉组织嗜酸粒细胞有促进凋亡的作用更多还原

【Abstract】 Objective To study the effect of erythromycin on apoptosis of eosinophils in nasal polyps in vitro Methods Fifteen nasal polyp biopsies were collected during nasal polypectomy The specimens were cultured with erythromycin in RPMI1640, and cultured without erythromycin as control group The explants in both groups were taken out at 1,3,5 day after culture The apoptosis of cells was detected by terminal deoxynucleotidyl transferasemediated dUTP nick endlabeling(TUNEL),eosinophils were identified by morphology and serial sections stained with HE, then the apoptosis index (AI) of eosinophils was calculated Results The AI of eosinophils in nasal polyps after cultured for 1,3,5 days with erythromycin were (31 77±9 52)%,(32 67±9 44)% and (50 34±8 78)% respectively In control group, the AI of eosinophils were (29 18±7 31)%,(29 82±12 03)% and (42 25±8 08)% respectively There were significant difference between two groups at 5 day after culture( P <0 05) Conclusion Erythromycin promoted apoptosis of eosinophils in nasal polyps in vitro更多还原