【摘要】 目的:研究金雀异黄素(genistein)对人乳腺癌细胞系的体外生长作用及其作用机理。方法:选用人乳腺癌细胞系MCF-7(雌激素受体阳性细胞,ER+)和MDA-MB-231(雌激素受体阴性细胞,ER-)体外培养、MTT比色法、生长曲线和雌激素受体(ER)免疫组化染色等。结果:genistein能明显地抑制MCF-7细胞和MDA-MB-231细胞的体外增殖,而且在一定浓度范围内均呈剂量依赖性,IC50分别是32.5,46.8μmol·L-1。同时能拮抗外源性雌激素对MCF-7细胞的生长刺激作用,而对MDA-MB-231细胞的生长抑制作用与雌激素的存在与否无关。ER免疫组化染色结果表明,经过30μmol·L-1 genistein处理的MCF-7细胞,细胞核内的阳性颗粒与对照组相比明显减弱(P<0.001)。结论:genistein能明显抑制MCF-7和MDA-MB-231细胞的体外生长,且呈剂量依赖性;genistein对MCF-7细胞的生长抑制作用是通过ER途径来介导的,而对MDA-MB-231细胞是通过非ER途径来介导的。更多还原

【Abstract】 Objective: To study the inhibitig effects of genistein on the growth of human breast cancer cell lines in vitro and its mechanisms. Methord: Human breast cancer cell lines both MCF-7(positive estrogen receptor, ER+ ) and MDA-MB-231(negative estrogen receptor, ER-) were cultured in vitro. The proliferation of cells was measured with MTT methord and the growth curve was drawn with cell count. The estrogen receptor in cells was show with immunohistochemistry. Result: Genistein inhibited proliferation of MCF-7 and MDA-MB-231 cell lines and inhibition was dependent on dose within some concentration range, IC50 being 32.5μmol·L-1 and 46. 8μmol·L-1 respectively. Genisteins antiproliferation of MCF-7 was stimulated by ectogenesis estrogen but proliferation of MDA-MB-231 inhibited by geinstein was not related to estrogen. The positive signs of ER in cellular nuclei of MCF-7 cell line fed with genistein at concentration of 30μmol·L-1 were significantly weaker than these not fed with genistein. Conclusion: Genistein obviously inhibits proliferation of both cell lines of MCF-7 and MDA-MB-231 in vitro and is dependent on dose. Genisteins antiproliferous effect on MCF-7 cell lines is stimulated by estrogen and this effect is related with ER, but genisteins inhibiting proliferation of MDA-MB-231 cell line is not through ER.更多还原