【摘要】 目的 :探讨IL - 6、氧自由基在急性胰腺炎 (acutepancreatitis,AP)合并肝损伤中的作用 ,及重组人白介素 - 2 (IL -2 )、川芎嗪的治疗价值。 方法 :SD大鼠 112只 ,随机分为 14组 ,每组 8只。 5 %牛磺胆酸钠逆行胰胆管内注射诱发大鼠AP动物模型 ,检测血浆IL - 6、SOD、MDA、ALT、AST、LDH、LIP、AMY ,并观察肝、胰病理变化。 结果 :①AP组血浆AMY、LIP、ALT、AST、LDH明显升高 (P <0 0 5或 0 0 1) ,镜下可见胰腺水肿、炎细胞浸润、坏死 ,肝脏肝窦充血、细胞浊肿及坏死 ,且损伤程度随时限延长而加重 ;②AP各组血浆IL - 6明显升高 (P <0 0 1) ;③AP各组MDA明显升高 (P <0 0 1)、SOD明显降低 (P <0 0 1)。④IL- 2治疗组、川芎嗪治疗组及IL - 2、川芎嗪联合应用组与NS组比较血浆IL - 6、MDA水平明显下降 (P <0 0 5 ) ,SOD明显升高(P <0 0 5 ) ,胰、肝病理损害程度减轻 ,并且AMY、LIP、ALT、AST、LDH均明显降低 (P <0 0 5 ) ,平均存活时间明显延长 (P <0 0 5 ) ;联合应用组降低IL - 6、MDA水平和减轻胰腺坏死优于单药组。 结论 :①IL - 6、氧自由基在急性胰腺炎合并肝损伤中明显升高 ,起损伤作用 ,而SOD明显降低 ,其保护作用减弱。检测血浆IL - 6、MDA、SOD可作为判断AP合并肝脏损伤程度和预后的指标更多还原

【Abstract】 Objective:To evaluate the effects of IL-6 and OFR in acute pancreatitis(AP)and the therapeutic mechanisms of IL-2,and tetratmetylpylazine(TMP) Methods:AP models were induced by retrograde injection of 5% sodium taurocholate into the pancreatic duct and serum levels of IL-6,SOD,MDA,ALT,AST,LDH,LIP,AMY were determined,and pathologic changes of the IL-6 and pancreas observed.Results:①In the AP group serum levels of AMY,LIP,ALT,AST,LDH increased significantly(P<0 05 or 0 01)and the pancreas and liver showed obvious pathologic changes.②In the AP group serum level of IL-6 increased significantly.③In the AP group serum level of MDA increased significantly.④After peritoneal injection of IL-2,TMP of IL-2+TMP,the serum levels of IL-6 and MDA decreased significantly and that of SOD increased,the liver and pancreatic pathologic damages score improved and the mean survival period prolonged,especially more marked in the combined IL-2+TMP group.Conclusion:①IL-6 and OFR play an important role in the pathogenesis of AP with liver damage,there is a positive correlation between them,so these parameters can be used as indicators to proedict the severity and prognosis of AP with liver damage.②IL-2 and TMP are of potentially therapeutic action to AP with liver damage,especially if combinedly used.更多还原