【摘要】 目的:对上皮性卵巢癌雌激素受体α、β亚型和孕激素受体A、B亚型表达情况进行分析,以了解其在卵巢癌中的作用。方法:选取1985年1月～2000年5月北京大学人民医院妇科收治的51例卵巢上皮性癌,10例卵巢良性上皮性肿瘤患者的石蜡病理切片,用SP-ABC法测定ERα、ERβ和PR-AB、PR-B蛋白表达情况,并与临床病理特征进行比较。结果:1)ERα、ERβ阳性染色均位于细胞核内。卵巢上皮性癌中,ERα(+)54.9%(28/51),与良性肿瘤接近(60.0%)。ERβ(+)90.2%(46/51),显著高于良性肿瘤(60.0%),ERβ阳性率在卵巢粘液癌、子宫内膜样癌以及中低分化癌中显著高于ERα(P<0.05)。2)PR-AB和PR-B亦为细胞核表达。良性肿瘤中PR-AB及PR-B均阳性90.0%。51例恶性肿瘤中PR-AB阳性11例(21.6%),PR-B阳性9例(17.6%),二者有显著性差异(P<0.001)。PR-AB和PR-B阳性表达在Ⅰ、Ⅱ期显著高于Ⅲ、Ⅳ期(P<0.01),在高分化者显著高于中、低分化者(P<0.05)。结论:ERα、ERβ和PR-AB与PR-B均为核受体。原发性上皮性卵巢癌中ERβ阳性率显著增高,可能为卵巢癌发生过程中的一个标志,PR-AB和PR-B高表达与肿瘤期别较早、分化较好有关,可提示预后,并可能与内分泌治疗有关。更多还原

【Abstract】 Objective:To study the role of ER,ER and PRA,PRB in epithelial ovarian tumors.Methods:Fifty-one cases of epithelial ovarian carcinoma and10cases of benign epithe-lial ovarian tumor were studied which were admitted to the People’s Hospital of Peking University during January1985to May2000.All patients did’nt receive chemotherapy or hormone treat-ment before operation.ERα,ERβand PRAB,PRB were analysed using immunohistochemistry in formalin-fixed paraffin-embedded tissue.The status of ERα,ERβ,and PRAB,PRB were com-pared to clinico-pathological characteristics of ovarian carcinoma.Results:1)ERαand ERβprotein were located in cellular nuclear.The positive expression rate of ERαwas54.9%(28/51)of primary epithelial ovarian carcinoma which was similar to the benign ovarian tumors(60.0%).The positive expression rate of ERβwas90.2%(46/51)of primary ovarian carcinoma which was significantly higher than those of benign tumors(60%).2)PR-AB and PR-B were also located in nuclear.The positive expression rate of PR-AB and PR-B of epithelial ovarian carcinoma were21.6%and17.6%respectively,which all were significantly lower than those of benign tu- mors(P<0.001).PR-AB and PR-B expression were obviously associated with early tumor stage(P<0.01)and well differentiation(P<0.05).Con clusion:The results show that ERα,ERβand PR-AB,PR-B are nuclear receptors and ERβhigh expression may be a useful marker of ovarian carcinogenesis.PR-AB and PR-B are associated with early stage and well differentiation.It could predict the prognosis and endocrinotherapy effect for ovarian carcinoma.更多还原