【摘要】 目的　建立血清中氟西汀的高效液相色谱检测方法 ,监测氟西汀血药浓度 ,为临床用药提供依据。方法　 0 .5mL样品用 2 %异丙醇的正庚烷 5mL提取液萃取净化。色谱条件 ,以C18柱 (4μm ,15 0mm× 3.9mm)作为分析柱 ,流动相为甲醇 磷酸氢二铵缓冲液 (70∶30 ) ,pH为 6 .4 ,流速为 0 .6mL·min-1,柱温 4 0℃ ,检测波长 2 30nm。结果　氟西汀在 0 .3～ 6 μmol·L-1范围内呈线性 ,回收率为 98.8%～ 10 1.4 % (n =5 )。结论　本法简便、快速、准确 ,适合于体液中氟西汀的监测及药动学研究更多还原

【Abstract】 OBJECTIVE To develop a HPLC method for the determination of fluoxetine in serum and provide a scientific basis for clinical use of fluoxetine.METHODS 0.5 mL serum sample was used.Doxepin hydrochloride was used as the internal standard.It was then extracted by 5 mL n heptane containing 2% iso proalcohol.C 18 column (4 μm,150 mm×3.9 mm) was used with the mobile phase of methanol sodium phosphate dibasic buffer solution (70∶30).The flow rate was 0.6 mL·min -1 and the column temparature was 40℃.The detection wavelength was 230 nm.RESULTS The linearity was obtained over the range of 0.3～6 μmol·L -1 .The lowest detection limit of fluoxetine in serum was 60 nmol·L -1 .The recoveries of three concentrations of fluoxetine in serum were 100.01%,99.93% and 99.93% respectively.CONCLUSION This report provided a rapid,convenient,sensitive and accurate method which is suitable for monitoring the concentrations of fluoxetine in serum and its pharmacokinetic study.更多还原