【摘要】 目的　用非水毛细管电泳法分离盐酸多塞平异构体。方法　采用未涂层石英毛细管柱 (50 μm× 47cm ,有效分离长度为 40cm) ,以用 2 0 0mmol·L- 1 醋酸铵的甲醇 乙腈 (95∶5)溶液 (含 3 .0 %冰醋酸 )为运行缓冲液 ,压力进样 ,时间 4s,运行电压为 2 5kV ,检测波长为 2 54nm。结果　方法分离效率高 ,精密度和回收率较好 ,所选浓度范围内线性关系良好 ,r值为 0 .9998。结论　方法简单可靠 ,分析时间短 ,可有效分离盐酸多塞平异构体更多还原

【Abstract】 OBJECTIVE To establish a method based on nonaqueo us capillary electrophoresis for the separation of Z ,E isomers of doxepine hydrochloride.METHODS The separation was performed on a fuse d silica capillary of 50 μm×47 cm (40 cm of effective length), and the runnin g buffer was methanol acetonitrile (95∶5) containing 200 mmol·L -1 ammon ium acetate and 3.0% glacial acetic acid.Samples were injected by pressure mode. The applied voltage was 25 kV and the detection wavelength was set at 254 nm.RESULTS The method was validated in terms of reproducibility,l inearity,recovery,limits of quantitation and detection.CONCLUSION The method appeared to be simple and fast for the separation of Z ,E isomers of doxepine hydrochloride.更多还原