【摘要】 自感染RHDV致死的兔肝脏组织中提纯病毒 ,提取病毒总RNA ,以RT_PCR方法扩增得到RHDVVP6 0基因全长片段 ,克隆到T载体中 ,经酶切分析及PCR鉴定后进行序列测定。同一毒株不同克隆产物进行 3次测序 ,测序结果已输送到GeenBank中 (注册号为AF45 376 1)。将测得序列与GeneBank中记录的RHDV不同分离株进行比较。比较结果表明 :各毒株间核苷酸的同源性为 91%～ 98% ,氨基酸同源性为 94%～ 99% ,氨基酸变异多发生在高变区 ,进一步证明了毒株的高度保守性 ,为明确我国RHDV地方株VP6 0蛋白的分子特征、分子诊断试剂及新型疫苗的研制奠定了基础更多还原

【Abstract】 Capsid protein VP60 is the only structure protein of RHDV(Rabbit Hemorrhagic Disease virus),which can induce immune response to RHDV.In this study,RHDV were purified from liver samples of rabbits killed by RHDV,and total RNA were repaired.A 1794bp and 2010 bp fragments of vp60 were amplified by RT-PCR then were cloned into pMD-T vector and sequenced.After 3 times sequencing of the different cloning products of the same RHDV-TP isolate,the obtained sequence has been submitted to GenBank with the accession number:AF453761.The nucleotide sequence and deduced amino acid sequence of RHDV-TP isolate were compared with other 15 strains of RHDV isolates form GenBank.The result shows that the nucleotide seuqence and deduced amino acid sequence of RHDV-TP isolate share the 91%～98% and 94%～99% homology with those isolates,respectively and amino acid diversion usually happened in variability region E and F.The results indicated a high level of conservation between isolates of RHDV and provided the possibility for molecule characteristic analysis of VP60 protein in China,molecule diagnoses reagent and new vaccine study of RHDV.更多还原