【摘要】 使用含 2 2 0 0条水稻表达序列标签的 c DNA微阵列检测了萌发期水稻胚芽、胚轴 (含糊粉层和盾片 )、胚根的基因表达模式 ,得到 10 32个有效的基因表达数据。其中 ,在胚芽、胚根、胚轴中特异表达的基因数分别为 5 5、10 6和 36个。以胚轴为参比 ,70个基因在胚芽、胚根中均表达上调 ,包括延伸因子 1和多个核糖体蛋白基因 ,这些基因与顶端生长点的细胞分化和生长有关。胚轴特异表达的基因有 36个 ,大部分参与胚乳贮藏物质的代谢 ,如聚泛蛋白基因、磷酸甘油酸激酶基因等更多还原

【Abstract】 cDNA microarray containing 2200 rice ESTs was used for monitoring the expression profiles of plumule, mesocotyl (including scutellum and aleurone tissue) and radicle in germinating rice seeds. One thousand and thirty two constitutively and differentially expressed genes were identified. Among them, 55, 106 and 36 genes were specifically expressed in plumule, radicle, and mesocotyl, respectively. Using mesocotyl as standard, 70 genes, including elongation factor 1 gene and several ribosomal protein genes, were up regulated expressed in both plumule and radicle, which contributed to cell differentiation and growth at apical points. Thirty six mesocotyl specific genes were mostly involved in the metabolism of storage materials in endosperm, such as polyubiquitin gene and phosphoglycerate kinase.更多还原