【摘要】 利用 RAPD标记对从体细胞突变体 HX- 3中鉴定出的抗水稻白叶枯病新基因 Xa- 2 5 (t)进行了研究 ,在 30 6个随机引物中 ,两个引物 S12 6 9和 S132 7在亲本和抗感池 (均由 12个高抗或高感植株组成 )之间表现多态性。用这两个引物对龙特甫 A和 HX- 3的 F2 群体的 114个单株进行连锁分析 ,表明 S12 6 9和 S132 7与 Xa- 2 5 (t)的连锁距离分别为 5 .3和 2 3.7c M,且位于 Xa- 2 5 (t)的两侧。更多还原

【Abstract】 Xa 25(t), a new resistance gene to bacterial blight identified from the somaclonal mutant HX 3, was studied by using RAPD marker. Out of the 306 random primers tested,two were found to be polymorphic between the parents and the resistant pool and susceptible pool(which were composed of 12 highly resistant plants and highly susceptible plants,respectively). With the two primers, 114 individuals from the F 2 population of Longtefu A/HX 3 were analyzed for linkage relationship. Results indicated that S1269 and S1327 were located on the opposite sides of Xa 25(t), with the distances of 5 3 cM and 23.7 cM respectively.更多还原