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酶法提取猪皮胶原及其结构表征
Preparation of pepsin-soluble collagen from pig skin and its characterization
【摘要】 利用胃蛋白酶提取猪皮胶原 ,并测定了所提取猪皮胶原的分子量、等电点、变性温度、紫外及红外吸收光谱 ,结果表明 :提取的猪皮胶原最大程度地保持了其天然结构 ,适合用作生物医学材料。
【Abstract】 Pepsin-soluble collagen was prepared from pig skin and partially characterized. The various physico-chemical properties, such as molecular weight, isoelectric point, denaturation temperature, UV-VIS and IR spectra of pig skin collagen were measured. As a result, the natural structure of pepsin-soluble collagen was maintained, therefore, it fits well for biomedical use.
【关键词】 胶原;
生物医学;
胃蛋白酶;
等电点;
变性温度;
【Key words】 collagen biomedical pepsin isoelectric point denaturation temperature;
【Key words】 collagen biomedical pepsin isoelectric point denaturation temperature;
【基金】 教育部“高等学校骨干教师资助计划”项目
- 【文献出处】 中国皮革 ,China Leather , 编辑部邮箱 ,2002年23期
- 【分类号】TS59
- 【被引频次】61
- 【下载频次】697