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不同梅毒螺旋体基因重组抗原酶联免疫试验比较

Comparison of Different Recombinant Antigen-Based Enzyme Immunoassays for Detection of Antibodies Against Treponema Pallidum

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【作者】 张瑞芬庄辉蒋志彬王薇媛杜兰英刘学恩

【Author】 ZHANG Rui fen,ZHUANG Hui,JIANG Zhi bin,et al.Department of Microbiology,School of Basic Medicine,Peking University(Beijing 100083,China)

【机构】 北京大学基础医学院微生物学教研室中国药品生物制品检定所北京大学基础医学院微生物学教研室 100083100083100083

【摘要】 目的 对单一梅毒螺旋体 (Treponemapallidum ,Tp)基因重组抗原Tp47、Tp42或Tp1 7及联合应用 3种基因重组抗原 (Tp47、Tp42和Tp1 7)酶联免疫试验 (enzymeimmunoassay,EIA)进行比较。 方法 分别用 3种单一Tp基因重组抗原Tp47、Tp42或Tp1 7EIA法 ,检测 1 1份梅毒血凝试验Tp抗体阳性和 2 8份阴性血清 ,并与联合应用 3种基因重组抗原 (Tp47、Tp42和Tp1 7)EIA法比较。 结果 单一基因重组抗原Tp47、Tp42或Tp1 7EIA法的灵敏度分别为1 0 0 % (1 1 / 1 1 )、1 0 0 % (1 1 / 1 1 )和 90 9% (1 0 / 1 1 ) ,特异度分别为 96 4% (2 7/ 2 8)、1 0 0 % (2 8/ 2 8)和 96 4% (2 7/ 2 8) ;联合应用 3种基因重组抗原EIA法的灵敏度和特异度分别为 1 0 0 % (1 1 / 1 1 )和 1 0 0 % (2 8/ 2 8)。结论 单一Tp基因重组抗原EIA法用于梅毒诊断存在一定的假阳性和假阴性反应 ,联合应用 3种基因重组抗原 (Tp47、Tp42和Tp1 7)EIA法的灵敏度高于单一Tp基因重组抗原EIA法

【Abstract】 Objective To compare the enzyme immunoassays (EIA) based on a single recombinant Treponema pallidum (TP) antigen Tp47,Tp42 or Tp17 with that using combination of the three recombinant Tp antigens.Methods Anti-Tp negaove and 10 anti-Tp positive sera tested by TPHA (Treponema pallidum hemagglutination) were detected by both the EIA based on combination of the 3 recombinant Tp antigens (Tp47,Tp42 and Tp17) and the EIA using a single Tp protein (Tp47,or Tp42,or Tp17).Results The sensitivity of EIA based on a single antigen Tp47,Tp42,or Tp17 was 100% (11/11),100% (11/11) and 90.9%(10/11),with the speciacity of 96 4% (27/28),100% (28/28) and 96 4% (27/28),respectively.The sensitivity and the specificity of EIA based on combination of the three Tp antigens were 100% (11/11) and 100% (28/28),respectively.Conclusion There was some false negative and false positive reactivity with the EIA based on a single recombinant antigen for diagnosis of syphilis.The sensitivity of EIA based on combination of the three antigens (Tp47,Tp42 and Tp17) for detection of anti-Tp antibody was higher than that of the EIA with a single recombinant Tp antigen.

【关键词】 梅毒基因重组抗原诊断酶联免疫试验
【Key words】 syphilisrecombinant antigendiagnosisEIA
  • 【文献出处】 中国公共卫生 ,China Public Health , 编辑部邮箱 ,2002年06期
  • 【分类号】R446.6
  • 【被引频次】5
  • 【下载频次】86
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