【摘要】 目的　探讨日本血吸虫 31/ 32kDa重组抗原 (rSj31/ 32 )抗血吸虫感染的免疫效果。方法　日本血吸虫 31/32kDa单克隆抗体免疫筛选的阳性克隆 312经IPTG诱导表达 ,表达产物rSj31/ 32加福氏佐剂免疫小鼠后进行攻击感染实验。结果　与对照组相比较 ,rSj31/ 32可诱导小鼠产生 30 1%的减虫率 ,6 3 2 %的减卵率 (P <0 0 1) ,虫卵肉芽肿平均面积和周长均明显降低 ,rSj31/ 32免疫能诱导一定水平的抗血吸虫抗体。 结论　结果表明 ,rSj31/ 32可诱导小鼠产生一定程度的保护免疫力 ,有可能作为血吸虫疫苗候选抗原分子。更多还原

【Abstract】 Objective To evaluate the immunoprotective effect of schistosoma japonicum recombinant 31/32kDa protein(rSj31/32).Methods S.japonicum adult worm cDNA expression library was screened with Sj31/32 McAb as a catching antibody probe.The positive clone 312 was introduced as lysogens into E.coli Y1089 and induced by IPTG.Mice were vaccinated with rSj31/32 at weeks 0,2,4 and challenged with 40±2 S.japonicum cercariae at weeks 5.Results The rSj31/32 could induce 30.1% worm reduction rate and 63.2% egg reduction rate (P<0.01).It could reduce the mean areas and perimeter of egg granuloma.Conclusion The results indicated that rSj31/32 could result in protection against a subsequent challenge of S.japonicum in mice.It might be used as a promising vaccine candidate of S.japonicunm.更多还原