【摘要】 目的　探讨补肾健脾中药健骨颗粒对去卵巢骨质疏松模鼠血清TNF α与TGF β1含量的变化。方法　切除卵巢建立大鼠骨质疏松模型 ,随机分成三组 ,分别喂服健骨颗粒、骨松宝、生理盐水 ,用药 12周后检测各组血清TNF α、TGF β1及E2 含量。结果　大鼠去卵巢后 12周 ,随着E2 水平下降 ,血清TNF α含量明显升高 ,而TGF β1浓度却明显降低。当喂服健骨颗粒 12周后 ,血清E2 水平和TGF β1浓度回升明显 ,而TNF α含量却出现下降 ,与同期生理盐水组相比有明显统计学意义。血清TGF β1、E2 含量与TNF α呈负相关 ,而TGF β1含量与E2 水平高度正相关。结论　E2 对TNF α有一定的抑制作用 ,而TGF β1的分泌可能需要雌激素等的刺激。健骨颗粒通过综合调整作用以提高模鼠E2水平 ,既制约了TNF α的合成、分泌及其活性 ,又解除了对TGF β1的抑制作用 ,使破骨细胞的骨吸收活动减弱 ,成骨细胞的骨形成作用增强 ,改善骨代谢的负平衡。更多还原

【Abstract】 Objective To investigate the effect of Jiangu granula(JGG)on serum TNF α and TGF β 1 of osteoporosis(OP)models of rats Methods OP model SD rats were produced by ovariectomy and were randomly divided into 3 group:normal saline group,Gusongbao treated group,JGG treated group.The serum levels of TNF α,TGF β 1 and E 2 were measured after 12 weeks of treatment by ELISA.Results The levels of serum TNF α increased at 12 weeks after operation,but serum TGF β 1 decreased.After treating with JGG 12 weeks the levels of serum TGF β 1 and E 2 increased,but serum TNF α decreased in comparison with the normal saline group.It was found that there were a negative correlation between TGF β 1,E 2 and TNF α,TGF β 1 and E 2 appeared in highly positive correlation.Conclusion E 2 inhibited serum TNF α,but the secretion of serum TGF β 1 relied on the E 2 stimulation.JGG raise the E 2 levels of OP model rats by comprehensive modulation;it not only inhibites the secretion,synthesis and activity of TNF α,but also relieves the inhibition of TGF β 1.Consequently JGG can inhibit the bone resorption of osteoclasts and accelerate the bone formation of osteoblasts,then improve the negative balance of bone metabolism.更多还原