【摘要】 目的 :探讨鱼精蛋白治疗恶性肿瘤的治疗机制。方法 :通过建立动物肿瘤模型 ,鱼精蛋白实验治疗 ,结合免疫组化及原位DNA缺口末端标记法 ,检测肿瘤内血管密度和凋亡指数。结果 :实验组血管密度明显低于对照组 (P <0 .0 1) ,同时观察到瘤细胞凋亡的现象 ,凋亡指数为 (7.6 7± 1.70 ) %。结论 :鱼精蛋白可明显降低肿瘤内血管密度 ,同时诱导细胞凋亡 ,治疗效果来自抑制血管生成和诱导瘤细胞凋亡的作用更多还原

【Abstract】 Purpose:To study the therapeutic mechanism through inducing apoptosis of tumour by protamine inhibiting angiogenesis . Methods:The vascular density and apoptotic index(AI)in the tumour were investigated by immunohistological and Tunel(terminal deoxynucletidy1 transferase-mediated dU TP nick end-labelling) methods in animal tumour model with experimental treatment. Results:The vascular density of tumour was much lower in controls. Apoptosis appeard in the treated group and AI is (7.67±1.70)%. Conclusions:Protamine could reduce the vascular density of tumour very much and induce the apoptosis of tumour cells. The therapeutic effects were achieved by inhibiting angiogenesis and inducing apoptosis of tumour.更多还原