【摘要】 目的　探讨凝血因子 基因在第六外显子 2 3384- 2 3387bp存在单核苷酸多态性 (SNP9)在我国北方人群脑血栓发病机制中的作用。方法　应用聚合酶链反应 -限制性片断长度多态性 (PCR- RFL P)的方法 ,检测 2 0例脑血栓患者和他们家系及 6 0例正常健康对照组凝血因子 第六外显子 2 3384- 2 3387bp多态性分布。结果　 2 0例脑血栓患者和 40例他们的家系成员 ,在 Mn II酶切后 ,可见 30 7bp片断基因类型全部是 A。正常 6 0例健康对照组基因多态性基因型也显示为 A,而在阳性对照可见 A、G和 AG。结论　在这一等位基因位点脑血栓家系和正常人群基因多态性基因型均为 A型。这一位点基因对我国脑血栓患者的作用机制有待于进一步探讨。更多还原

【Abstract】 Objective This study is targeted for determing the frequency of SNP9 of coagulation factor-Ⅸ which exists in exon 6 betweet 23384 23387bp in cerebral thrombosis patients and direct tran generati in relatives of them from northeastern China. Methods Restriction Fragment Length Polymorphism(RFLP) was applied in SNP9 genotyping in 20 patients and 40 relatives of the patients,as well as 60 random healthy individuals as control. Results SNP9 genotype of 307bp fragment was all A type after dissolved by MnII enzyme in 20 patients with cerebral thrombosis,40 members of their families and 60 healthy people. A type,G type and AG type were found in positive contrastivepeople. Conclusion There is no difference between alleles at SNP9 of cerebral thrombosis patients and those of control.更多还原