【摘要】 目的:利用PE-377型DNA测序仪对来自不同染色体上的微卫星D3S1358、vWA、FGA、D8S1179、D21S11、D18S51、D5S818、D13S317和D7S820共9个位点及性别位点Amelogenin进行基因型检测,并探讨其在异基因造血干细胞移植中作为供者造血干细胞植活证据的可行性。方法:用多重PCR和四色荧光自动化检测技术分析9个微卫星位点,并比较供者和受者移植前、后的基因型。结果和结论:本研究所选用的9个微卫星位点可作为异基因造血干细胞移植中供者干细胞植活的证据。更多还原

【Abstract】 Objective:To search engraft evidence in allogeneic haematopoietic stem cell transplantation using ABI PRISM 377 by geno-typing 9 microsatellite loci for D3S1358, vWA , FGA, D8S1179, D21S11, D18S51, D5S818, D13S317, D7S820 and sexual locus Amelogenin. Methods: Multiplex PCR and GeneScan methods were used to genotype sample DNA. The results of the donor and the recipient in pre-transplantation and post-transplantation were analyzed. Results and Conclusions: Genotyping of the nine microsatellite loci could be used as engraft evidence in post-transplantation.更多还原