【摘要】 目的 :建立了通督活血片的质量标准。方法 :采用TLC对处方中丹参、延胡索进行鉴别 ;用HPLC测定丹参酮ⅡA的含量 ,双波长薄层扫描法测定黄芪甲苷的含量。结果 :在TLC色谱中均能检出丹参、延胡索 ;丹参酮ⅡA 和黄芪甲苷含量测定方法的线性范围分别为 0 .0 4～ 0 .8μg(r =0 .99998,n =7)和 0 .3～ 6 .0 μg(r =0 .9991,n =7) ,平均回收率分别为98.6 % (RSD =1.1% ,n =5 )和 97.7% (RSD =1.8% ,n =5 ) ,含量限度丹参酮ⅡA 不少于 0 .4 0mg·片-1,黄芪甲苷不少于0 .18mg·片 -1。结论 :所建立的方法准确可行 ,重复性好 ,可作为通督活血片质量控制标准更多还原

【Abstract】 Objective:To establish the quality standard for Tongduhuoxue Tablets.Methods: Radix Salvia miltiorrhiza and Rhizoma Corydalis in this prescription were identified by TLC. Tanshinone Ⅱ A was determined by HPLC and astragaloside was determined by dual wavelength TLC Scanning.Results:Radix Salvia miltiorrhiza and Rhizoma Corydalis could be detected by TLC. Determination method of tanshinone Ⅱ A and astragaloside was linear at the range of 0.04 ～ 0.8 μg( r= 0.99998 ,n=7 ) and 0.3 ～ 6.0 μg( r= 0.9991 ,n=7 ),average recovery was 98.6% ( RSD= 1.1% ,n=5 ) and 97.7% ( RSD= 1.8% ,n=5 ),respectively. Conclusions: The established method is accurate and reproducible. This study provides a method for the quality control of Tongduhuoxue Tablets.更多还原